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Apaf-1 monoclonal antibody (18H2)

 
ALX-804-350-C100 100 µg 334.00 USD
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Product Specification

Alternative Name:Apoptosis protease-activating factor 1
 
Clone:18H2
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse Apaf-1 (apoptosis protease-activating factor-1) (aa 1-97) containing the N-terminal CARD domain.
 
UniProt ID:O88879
 
Species reactivity:Human, Mouse
 
Specificity:Recognizes the CARD domain of Apaf-1.
 
Crossreactivity:Does not cross-react with rat Apaf-1.
 
Applications:ELISA, ICC, IP, WB
 
Formulation:Liquid. Protein G purified antibody in PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-350-n1
Figures: Species cross-reactivity of MAb to Apaf-1 (clone 18H2) (ALX-804-350) by Western blot.A: Lysates from 1-day-old mouse brain (lane 1), adult mouse brain (lane 2) and SH-SY5Y human neuroblastoma cell line (lane 3) were probed with clone 18H2.B: Lysates from 2-days-old rat brain (lane 1) and adult rat brain (lane 2) were probed with clone 18H2. Results: 18H2 is human and mouse specific but does not recognize rat Apaf-1.
804-350
Figure: Species cross-reactivity of MAbs to APAF-1 by WB. left: Lysates from 1-day-old mouse brain (lane 1), adult mouse brain (lane 2) and SH-SY5Y human neuroblastoma cell line (lane 3). right: Lysates from 2-days-old rat brain (lane 1) and adult rat brain (lane 2).
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804-350-n1 804-350

Product Literature References

Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood 107, 1872 (2006), Abstract; Full Text
Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation: C.L. Scott, et al.; J. Cell Biol. 164, 89 (2004), Abstract; Full Text
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000), Abstract; Full Text

General Literature References

Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci. 58, 1688 (2001), (Review), Abstract;
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell. Biol. 149, 623 (2000), Abstract; Full Text
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell 94, 727 (1998), Abstract;
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS 95, 4386 (1998), Abstract; Full Text
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997), Abstract;
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997), Abstract;

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