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Apaf-1 monoclonal antibody (13F11)

 
ALX-804-349-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:Apoptosis protease-activating factor 1
 
Clone:13F11
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse Apaf-1 (apoptosis protease-activating factor 1) (aa 1-97) containing the N-terminal CARD domain.
 
UniProt ID:O88879
 
Species reactivity:Mouse, Rat
 
Specificity:Recognizes the CARD domain of Apaf-1.
 
Crossreactivity:Does not cross-react with human Apaf-1.
 
Applications:ICC, IP, WB
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-349-n2
Figures: Species cross-reactivity of MAb to Apaf-1 (clone 13F11) (ALX-804-349) by Western blot.
A: Lysates from 1-day-old mouse brain (lane 1), adult mouse brain (lane 2) and SH-SY5Y human neuroblastoma cell line (lane 3) were probed with clone 13F11.
B: Lysates from 2-days-old rat brain (lane 1) and adult rat brain (lane 2) were probed with clone 13F11.
Results: 13F11 is mouse and rat specific but does not recognize human Apaf-1.
804-349-n1
Figure: Western blot analysis with anti-Apaf-1 MAb clone 13F11 on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1- mice.
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Product Literature References

Inhibition of apoptosome formation by suppression of Hsp90beta phosphorylation in tyrosine kinase-induced leukemias: M. Kurokawa, et al.; Mol. Cell Biol. 28, 5494 (2008), Abstract; Full Text
Essential postmitochondrial function of p53 uncovered in DNA damage-induced apoptosis in neurons: A.E. Vaughn & M. Deshmukh; Cell Death Differ. 14, 973 (2007), Abstract; Full Text
Decreased apoptosome activity with neuronal differentiation sets the threshold for strict IAP regulation of apoptosis: K.M. Wright, et al.; J. Cell Biol. 167, 303 (2004), Abstract; Full Text

General Literature References

Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci. 58, 1688 (2001), (Review), Abstract;
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000), Abstract; Full Text
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell 94, 727 (1998), Abstract;
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS 95, 4386 (1998), Abstract; Full Text
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997), Abstract;
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997), Abstract;

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