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Apaf-1 (human) monoclonal antibody (2E12)

 
ALX-804-348-C100 100 µg 323.00 USD
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Product Specification

Alternative Name:Apoptosis protease-activating factor 1
 
Clone:2E12
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant human Apaf-1 (apoptosis protease-activating factor 1) N-terminal CARD and CED-4 homologous domains (aa 1-464) .
 
UniProt ID:O14727
 
Species reactivity:Human
 
Specificity:Recognizes the CARD domain of Apaf-1.
 
Crossreactivity:Does not cross-react with mouse or rat Apaf-1.
 
Applications:ELISA, ICC, IHC (FS), IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (EM on frozen sections; for details see G. Hausmann, et al., below.)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-348-n1
Figure: Western blot analysis with anti-Apaf-1 (human) MAb (clone 2E12) revealing endogenous Apaf-1. Lysates from 106 HeLa, MCF-7, 293T, COS, Jurkat, and activated primary human T cells were probed with clone 2E12. COS (monkey) cells were used as a negative control.
804-348-n2
Figures: Species cross-reactivity of MAb to Apaf-1 (clone 2E12) (ALX-804-348) by Western blot.
A: Lysates from 1-day-old mouse brain (lane 1), adult mouse brain (lane 2) and SH-SY5Y human neuroblastoma cell line (lane 3) were probed with clone 2E12.
B: Lysates from 2-days-old rat brain (lane 1) and adult rat brain (lane 2) were probed with clone 2E12.

Results: 2E12 is human specific and does not cross-react with mouse or rat Apaf-1.
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804-348-n1 804-348-n2

Product Literature References

BOK and NOXA Are Essential Mediators of p53-dependent Apoptosis: A.G. Yakovlev, et al.; J. Biol. Chem. 279, 28367 (2004), Abstract;
Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein: D.H. Cho, et al.; J. Biol. Chem. 279, 39942 (2004), Abstract; Full Text
Anti-Fas antibody-induced apoptosis and its signal transduction in human gastric carcinoma cell lines: K. Adachi, et al.; Int. J. Oncol. 23, 713 (2003), Abstract;
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000), Abstract; Full Text
Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1: K. Moriishi, et al.; PNAS 96, 9683 (1999), Abstract; Full Text

General Literature References

Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci. 58, 1688 (2001), (Review), Abstract;
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell 94, 727 (1998), Abstract;
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS 95, 4386 (1998), Abstract; Full Text
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997), Abstract;
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997), Abstract;

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