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Toll-like receptor 2 (human) monoclonal antibody (TL2.1) (FITC conjugate)

ALX-804-323F-T100 100 tests FITC 309.00 USD
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Protected by US Patent no. US 7,071,310. License rights on the Patent are acquired from Leiv Eiriksson Nyskaping AS, Norway.
Enzo Life Sciences is not responsible for any patent infringements that might result with the use or derivation of this product.

Product Specification

Alternative Name:TIL4, Toll/Interleukin-1 receptor-like gene 4, TLR2, CD282
Immunogen:CHO cells stably transfected with human TLR2 (Toll-like receptor 2).
UniProt ID:O60603
Species reactivity:Human
Crossreactivity:Does not cross-react with human TLR1 or TLR4.
Applications:Flow Cytometry
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
Handling:Do not freeze. Protect from light.
Shipping:Blue Ice Not Frozen
Short Term Storage:+4°C
Long Term Storage:+4°C
Technical Info/Product Notes:1 test means: 1µl of a 0.1mg/ml stock solution were used to stain 200’000 cells in a sample volume of 50µl.
Figure: FACS analysis using MAb to TLR2 (human) (TL2.1) (FITC) (Prod. No. ALX-804-323F) to detects humanTLR2.Method: THP-1  cells (2 x 105) were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µl MAb to TLR2 (human) (TL2.1) (FITC) (Prod. No. ALX-804-323F) or a matched isotype-FITC control, washed and then analyzed by flow cytometry.
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Product Literature References

The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies: N. Satta, et al.; Blood 109, 1507 (2007), Abstract;
Divergent trophoblast responses to bacterial products mediated by TLRs: V.M. Abrahams, et al.; J. Immunol. 173, 4286 (2004), Abstract;
Haemophilus influenzae porin induces Toll-like receptor 2-mediated cytokine production in human monocytes and mouse macrophages: M. Galdiero, et al.; Infect. Immun. 72, 1204 (2004), Abstract; Full Text
A novel host-parasite lipid cross-talk. Schistosomal lyso-phosphatidylserine activates toll-like receptor 2 and affects immune polarization: D. van der Kleij, et al.; J. Biol. Chem. 277, 48122 (2002), Abstract; Full Text
Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells: A.E. Medvedev, et al.; J. Immunol. 169, 5209 (2002), Abstract; Full Text
Lipopolysaccharide rapidly traffics to and from the Golgi apparatus with the toll-like receptor 4-MD-2-CD14 complex in a process that is distinct from the initiation of signal transduction: E. Latz, et al.; J. Biol. Chem. 277, 47834 (2002), Abstract; Full Text
Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responses: I. Sabroe, et al.; J. Immunol. 168, 4701 (2002), Abstract;
Two lipoproteins extracted from Escherichia coli K-12 LCD25 lipopolysaccharide are the major components responsible for Toll-like receptor 2-mediated signaling: H.K. Lee, et al.; J. Immunol. 168, 4012 (2002), Abstract; Full Text
A novel synthetic acyclic lipid A-like agonist activates cells via the lipopolysaccharide/toll-like receptor 4 signaling pathway: E. Lien, et al.; J. Biol. Chem. 276, 1873 (2001), Abstract; Full Text
Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation: E. Faure, et al.; J. Immunol. 166, 2018 (2001), Abstract; Full Text
Differential expression of Toll-like receptor 2 in human cells: T.H. Flo, et al.; J. Leukoc. Biol. 69, 474 (2001), Abstract;
Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae: J.E. Wang, et al.; Infect. Immun. 69, 2402 (2001), Abstract; Full Text
Monocytic cell activation by Nonendotoxic glycoprotein from Prevotella intermedia ATCC 25611 is mediated by toll-like receptor 2: S. Sugawara, et al.; Infect. Immun. 69, 4951 (2001), Abstract;
Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide: T.H. Flo, et al.; J. Immunol. 164, 2064 (2000), Abstract; Full Text
Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products: E. Lien, et al.; J. Biol. Chem. 274, 33419 (1999), Abstract; Full Text

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