With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06µg/l have been obtained. The provisional guideline value proposed by the WHO is 1 µg/l for drinking water. The detection limit for microcystin-LR is 0.006 µg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x1010 l/mol.The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1µg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
Product Details
| Clone: | MC10E7 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes all 4-Arg microcystins. |
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| Applications: | ELISA, IHC (PS), WB
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| Recommended Dilutions/Conditions: | ELISA (1:10,000-1:100,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Microcystin concentrations can be measured in the range of ~0.006µg/l to 0.3µg/l. The ELISA sensitivity is about 1’000 times higher compared to UV detection. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Lyophilized. |
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| Reconstitution: | Reconstitute with 200µl PBS containing sodium azide. |
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| Use/Stability: | Storage at 4°C will decrease antibody activity and must be avoided. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Acteoside from Conandron ramondioides Reduces Microcystin-LR Cytotoxicity by Inhibiting Intracellular Uptake Mediated by OATP1B3: S. Takumi, et al.; Planta Med.
89, 616 (2023),
Abstract;
Microcystin-leucine arginine (MC-LR) induces mouse ovarian inflammation by promoting granulosa cells to produce inflammatory cytokine via activation of cGAS-STING signaling: K. Liu, et al.; Toxicol. Lett.
358, 6 (2022),
Abstract;
Diel Variations of Extracellular Microcystin Influence the Subcellular Dynamics of RubisCO in Microcystis aeruginosa PCC 7806: A. Guljamow, et al.; Microorganisma
9, 1265 (2021),
Abstract;
Roles of piRNAs in microcystin-leucine-arginine (MC-LR) induced reproductive toxicity in testis on male offspring.: L. Zhang, et al.; Food Chem. Toxicol.
105, 177 (2017),
Abstract;
Intraperitoneal exposure of whitefish to microcystin-LR induces rapid liver injury followed by regeneration and resilience to subsequent exposures: M. Wozny, et al.; Toxicol. Appl. Pharmacol.
313, 68 (2016),
Application(s): Immunohistochemical staining and Western Blot, tissue sections,
Abstract;
Microcystin-LR promotes cell proliferation in the mice liver by activating Akt and p38/ERK/JNK cascades: J. Liu, et al.; Chemosphere
163, 14 (2016),
Application(s): Mouse injection, intraperitoneally with 20, 40, and 80 μg/kg/d for 2 h, 1 d, 2 d, and 4 d,
Abstract;
Occurrence of microcystins in water, bloom, sediment and fish from a public water supply: F. Gurbuz, et al.; Sci. Total Environ.
562, 860 (2016),
Application(s): Determination of extractable MCs in fish tissue,
Abstract;
Reproductive toxicity on female mice induced by microcystin-LR: J. Wu, et al.; Environ. Toxicol. Pharmacol.
37, 1 (2013),
Application(s): Western blot,
Abstract;
Microcystin-LR induces cytoskeleton system reorganization through hyperphosphorylation of tau and HSP27 via PP2A inhibition and subsequent activation of the p38 MAPK signaling pathway in neuroendocrine (PC12) cells: G. Meng, et al.; Toxicology
290, 218 (2011),
Abstract;
Localization of microcystin-LR in medaka fish tissues after cyanotoxin gavage: C. Djediat, et al.; Toxicon
55, 531 (2010),
Abstract;
Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey: F. Gurbuz, et al.; Sci. Total Environ.
407, 4038 (2009),
Abstract;
Characterization of organic anion transporting polypeptide 1b2-null mice: essential role in hepatic uptake/toxicity of phalloidin and microcystin-LR: H. Lu, et al.; Toxicol Sci.
103, 35 (2008),
Abstract;
Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci.
17, 1445 (2001),
Abstract;
Highly sensitive immunoassay based on a monoclonal antibody specific for [4-arginine]microcystins: A. Zeck, et al.; Anal. Chim. Acta 441, 1 (2001),
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