Microcystin-LR, mAb (MC10E7)



ALX-804-320-C200	200 µg	228.00 USD

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC₅₀ values for microcystin-LR of 0.06µg/l have been obtained. The provisional guideline value proposed by the WHO is 1 µg/l for drinking water. The detection limit for microcystin-LR is 0.006 µg/l. All microcystin variants containing an arginine at position 4 show similar IC₅₀ values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x10¹⁰ l/mol.The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable.

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Product Specification

Purity Detail:	Protein G-affinity purified.

Purity:	≥95% (FPLC)

Formulation:	Lyophilized.

Clone:	MC10E7

Isotype:	Mouse IgG1

Immunogen:	Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.

Reconstitution:	Reconstitute with 200µl PBS containing sodium azide.

Specificity:	Recognizes all 4-Arg microcystins.

Application:	ELISA (1:10’000-1:100’000) Immunohistochemistry (paraffin sections) Microcystin concentrations can be measured in the range of ~0.006µg/l to 0.3µg/l. The ELISA sensitivity is about 1’000 times higher compared to UV detection. Western blot

Long Term Storage:	-20°C

Use/Stability:	Storage at 4°C will decrease antibody activity and must be avoided.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.

Product Literature References

Microcystin-LR induces cytoskeleton system reorganization through hyperphosphorylation of tau and HSP27 via PP2A inhibition and subsequent activation of the p38 MAPK signaling pathway in neuroendocrine (PC12) cells: G. Meng, et al.; Toxicol. in press, (2011), Abstract;

Localization of microcystin-LR in medaka fish tissues after cyanotoxin gavage: C. Djediat, et al.; Toxicon 55, 531 (2010), Abstract;

Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey: F. Gurbuz, et al.; Sci. Total Environ. 407, 4038 (2009), Abstract;

Characterization of organic anion transporting polypeptide 1b2-null mice: essential role in hepatic uptake/toxicity of phalloidin and microcystin-LR: H. Lu, et al.; Toxicol Sci. 103, 35 (2008), Abstract;

Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci. 17, 1445 (2001), Abstract;

Highly sensitive immunoassay based on a monoclonal antibody specific for [4-arginine]microcystins: A. Zeck, et al.; Anal. Chim. Acta 441, 1 (2001),