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TRAIL-R1 (human) monoclonal antibody (HS101) (FITC conjugate)

ALX-804-297F-T100 100 tests FITC 443.00 USD
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Product Specification

Alternative Name:TRAIL receptor 1, Apo-2, CD261, DR4, Death receptor 4, TNFRSF 10A, TNF-related apoptosis-inducing ligand receptor 1, Tumor necrosis factor receptor superfamily member 10A
Immunogen:Recombinant human TRAIL-R1.
UniProt ID:O00220
GenBank ID:U90875
Gene/Protein Identifier:8797 (Entrez GeneID)
Species reactivity:Human
Crossreactivity:Does not cross-react with human TRAIL-R2, -R3 or -R4.
Applications:Flow Cytometry, ICC
Recommended Dilutions/Conditions:Flow Cytometry (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. 50µg in PBS containing 0.09% sodium azide.
Handling:Do not freeze. Protect from light.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:+4°C
Figure: Flow cytometric detection of endogenous TRAIL-R1 surface expression on BJAB cells versus a TRAIL-R1 negative cell line, Jurkat.Method: BJAB or Jurkat cells (1x106) were stained with FITC-conjugated monoclonal antibody (MAb) HS101 against TRAIL-R1 (Prod. No. ALX-804-297F). Antibodies were used at 5µg/ml.
Figure: Receptor specifitiy of the FITC-conjugated MAb HS101 for TRAIL-R1 (Prod. No. ALX-804-297F).
Method: CV1/EBNA cells were transfected either with the empty expression vector pCDNA3 (vector control) or pCDNA3 encoding TRAIL-R1 to TRAIL-R4. Two days after transfection 1x106 cells were stained with FITC-conjugated MAb HS101 against TRAIL-R1. All primary antibodies were used at 5µg/ml. Transfection efficiency was about 50% in all individual transfections.
Figure A: Strong signal intensity is obtained by FITC-conjugated MAb HS101 (Prod. No. ALX-804-297F) compared to FITC-conjugated control.Method: CV1/EBVA cells (1x106) transiently transfected with pCDNA3 encoding  TRAIL-R1, were incubated with FITC-conjugated MAb HS101 against TRAIL-R1 or FITC-conjugated matched isotype control (mouse IgG1). Antibodies were used at 5µg/ml.Technical Note: Signal intensity is comparable to a secondary (anti-mouse IgG1-FITC) antibody (data not shown).Figure B: Signal intensity achieved with  FITC-conjugated MAb HS101 compared to  additional biotin and streptavidin-PE fluorochrome amplification step.Method: CV1/EBVA cells were either transiently transfected with pCDNA3 encoding  TRAIL-R1 (black histogram) or with empty expression vector pCDNA3 (white histogram). Cells (1x106) were first incubated with FITC-conjugated MAb HS101 (5µg/ml), followed by biotinylated anti-mouse IgG1 and streptavidin-PE.Technical Note: Signal increase is not significantly greater for biotin and streptavidin-PE amplification as opposed to FITC-conjugated MAb HS101 alone (Prod. No. ALX-804-297 for additional information).
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Product Literature References

Decreased affinity of recombinant human tumor necrosis factor-related apoptosis-inducing ligand (rhTRAIL) D269H/E195R to osteoprotegerin (OPG) overcomes TRAIL resistance mediated by the bone microenvironment: M.C. Bosman, et al.; J. Biol. Chem. 289, 1071 (2014), Abstract; Full Text
JNK inhibition sensitises hepatocellular carcinoma cells but not normal hepatocytes to the TNF-related apoptosis-inducing ligand: S.R. Mucha, et al.; Gut 58, 688 (2009), Application(s): Flow cytometry of human liver cancer cells, Abstract;
Resistance to FasL and tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in Sezary syndrome T-cells associated with impaired death receptor and FLICE-inhibitory protein expression: E. Contassot, et al.; Blood 111, 4780 (2008), Abstract;
P60-c-src suppresses apoptosis through inhibition of caspase 8 activation in hepatoma cells, but not in primary hepatocytes: E. De Toni, et al.; J. Hepatol. 46, 682 (2007), Application(s): Flow cytometry of human liver cancer cells, Abstract;
Casein kinase I attenuates tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by regulating the recruitment of fas-associated death domain and procaspase-8 to the death-inducing signaling complex: K. Izeradjene, et al.; Cancer Res. 64, 8036 (2004), Abstract; Full Text
cFLIPL inhibits tumor necrosis factor-related apoptosis-inducing ligand-mediated NF-kappaB activation at the death-inducing signaling complex in human keratinocytes: T. Wachter, et al.; J. Biol. Chem. 279, 52824 (2004), Abstract; Full Text
Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs: T.M. Ganten, et al.; Cell Death Differ. 11 Suppl 1, S86 (2004), Abstract;
IFN{alpha}-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells: C. Tecchio, et al.; Blood 103, 3837 (2004), Abstract;
TRAIL regulates normal erythroid maturation through an ERK-dependent pathway: P. Secchiero, et al.; Blood 103, 517 (2004), Abstract;
TRAIL-related death receptors in normal, Lurcher and weaver mutant mouse brain: J. Baurle, et al.; Neurosci. Lett. 372, 46 (2004), Abstract;
Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation: M. Leverkus, et al.; Mol. Cell. Biol. 23, 777 (2003), Abstract; Full Text
Silencing of death receptor and caspase-8 expression in small cell lung carcinoma cell lines and tumors by DNA methylation: S. Hopkins-Donaldson , et al.; Cell Death Differ. 10, 356 (2003), Application(s): Flow and WB of human lung carcinoma cells, Abstract; Full Text
TNF-Related Apoptosis-Inducing Ligand Mediates Tumoricidal Activity of Human Monocytes Stimulated by Newcastle Disease Virus: B. Washburn, et al.; J. Immunol. 170, 1814 (2003), Abstract;
Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8: M.R. Sprick, et al.; EMBO J. 21, 4520 (2002), Abstract;
Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Signaling in Activated T Cells Abrogates TRAIL-Induced Apoptosis Upstream of the Mitochondrial Amplification Loop and Caspase-8: T.S. Soderstrom, et al.; J. Immunol. 169, 2851 (2002), Abstract;

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