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TRAIL (human) monoclonal antibody (2E5)

 
ALX-804-296-C100 100 µg 367.00 USD
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Product Specification

Alternative Name:Apo-2L, TNFSF 10, CD253
 
Clone:2E5
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human soluble TRAIL (aa 95-281).
 
UniProt ID:P50591
 
GenBank ID:U57059
 
Gene/Protein Identifier:8743 (Entrez GeneID)
 
Species reactivity:Human
 
Crossreactivity:Does not cross-react with mouse TRAIL.
 
Applications:Flow Cytometry, FUNC
 
Application Notes:Functional Application (excellent): neutralizes human TRAIL mediated killing in a concentration range of 0.5-1.5µg/ml.
Only detects the native protein. Does not work in WB, ICC, IHC or other applications where fixation is necessary.
 
Purity Detail:Protein A-affinity purified. Sterile filtered.
 
Formulation:Liquid. In PBS, pH 7.4. Contains no preservatives.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
804-296-n1
Figure 1: Apoptosis induced in Jurkat T leukemia cells by KillerTRAILTM (Prod. No. ALX-201-073) is completely blocked by mouse MAb to TRAIL (human) (2E5) (Prod. No. ALX-804-296) in a concentration range of 0.5-1.5µg/ml. Concentration of MAb 2E5 required for inhibition may vary depending of the cell type studied and the concentration of soluble recombinant human TRAIL used. The neutralizing activity of MAb 2E5 has been confirmed with various sources of soluble recombinant human TRAIL including KillerTRAILTM.Method: Jurkat cells (5x104) were treated with KillerTRAILTM (300ng/ml) in the presence or absence of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analyzed by flow cytometry.
804-296-n2
Figure 2:  MAb to TRAIL (human) (2E5) (Prod. No. ALX-804-296) can be used to detect surface human TRAIL in transiently transfected 293T cells in flow cytometry as well on untransfected cells expressing endogenous surface TRAIL.Method: 293T cells (10cm dishes) were transfeced with 0, 1, or 15µg plasmid coding for full length human TRAIL for 24 hours using a standard calcium phosphate transfection method. All dishes were cotransfected with 1µg plasmid coding for EGFPas a cotransfection marker. Viable cells (FSC/SSC region R1), that were GFP-positive (transfected) (FL1 region R3) were analyzed with respect to surface expression of human TRAIL. Cells were stained with MAb to TRAIL (human) (2E5) as well as with an isotype-matched control (control IgG1) at 10µg/ml.  Binding of the primary MAbs is detected using RPE conjugated goat anti-mouse IgG (FL2).
804-296-n3
Figure: Neutralizing effect of MAb to TRAIL (human) (2E5) on TRAIL-induced apoptosis (TRAIL, Soluble (human) (recombinant) Set (Prod. No. ALX-850-018); containing Enhancer for Ligands).
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Product Literature References

A Bak-dependent mitochondrial amplification step contributes to Smac mimetic/glucocorticoid-induced necroptosis: K. Rohde, et al.; Cell Death Differ. (2016), Abstract;
Inhibition of B-Raf/MEK/ERK signaling suppresses DR5 expression and impairs response of cancer cells to DR5-mediated apoptosis and T cell-induced killing: Y.T. Oh, et al.; Oncogene 35, 459 (2016), Application(s): Functional Studies, Abstract;
IFN{alpha}-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells: C. Tecchio, et al.; Blood 103, 3837 (2004), Abstract;
TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors: M. Plasilova, et al.; Leukemia 16, 67 (2002), Abstract;

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