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Rab 9 monoclonal antibody (Mab9)

 
ALX-804-286-R100 100 µl 562.00 USD
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Product Specification

Clone:Mab9
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant dog Rab 9.
 
UniProt ID:P24408
 
Species reactivity:Human, Rat
Bovine, Dog, Hamster, Monkey
 
Crossreactivity:Does not cross-react with other Rab family members.
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:500),Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~26kDa by Western blot.
 
Formulation:Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components.

It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

 

General Literature References

Rab GTPases, directors of vesicle docking: F. Schimmöller, et al.; J. Biol. Chem. 273, 22161 (1998), Abstract; Full Text
Quantitative analysis of the interactions between prenyl Rab9, GDP dissociation inhibitor-alpha, and guanine nucleotides: A.D. Shapiro & S.R. Pfeffer; J. Biol. Chem. 270, 11085 (1995), Abstract; Full Text
Rab GDI: a solubilizing and recycling factor for rab9 protein: T. Soldati, et al.; Mol. Biol. Cell 4, 425 (1993), Abstract;

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