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FasL monoclonal antibody (3C82)

 
ALX-804-273-C100 100 µg 430.00 USD
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Product Specification

Alternative Name:CD95L, APO-1L, CD178, TNFSF 6
 
Clone:3C82
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human FasL (APO-1L; CD95L; CD178) (extracellular domain).
 
UniProt ID:P48023
 
Species reactivity:Human, Mouse
 
Applications:Flow Cytometry, FUNC
 
Recommended Dilutions/Conditions:Suggested starting dilution is >1:100
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Functional Application: neutralizing
 
Purity Detail:Epitope-affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 1% BSA. Contains no preservatives.
 
Handling:Do not freeze.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
804-273
Schematic structure of recombinant human FasL (extracellular domain).
804-273-1
Figure: Inhibition of mouse FasL (supernatant of FasL-transfected Neuro2A cells) by 3C82.Method: Supernatants of Neuro2A cells containing murine FasL (1:2 dilution) were incubated with Fas ecpressing L929-APO-1 target cells. After 14h apoptosis was assessed by MTT staining.
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Product Literature References

Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract; Full Text

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