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E-cadherin (mouse) monoclonal antibody (ECCD-1)

 
ALX-804-263-C100 100 µg 443.00 USD
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Product Specification

Alternative Name:Uvomorulin, CD324, Epithelial cadherin, Cadherin-1
 
Clone:ECCD-1
 
Host:Rat
 
Isotype:IgG2b
 
Immunogen:Mouse teratocarcinoma cell line F9.
 
UniProt ID:P09803
 
Source:Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of Wistar rat immunized with mouse teratocarcinoma cell line F9. Purified from serum-free cell culture supernatant.
 
Species reactivity:Mouse
 
Specificity:Epitope is close to the N-terminus of E-cadherin.
 
Crossreactivity:Does not cross-react with human, bovine, chicken or dog E-cadherin.
 
Applications:FUNC
 
Recommended Dilutions/Conditions:Functional Application (200µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Functional Application: inhibits E-cadherin-dependent cell-cell contact.
 
Purity Detail:Affinity purified.
 
Formulation:Lyophilized from affinity purified antibody, dissolved in 10mM PBS, pH 7.4, containing 1.0% BSA. (contains no preservatives)
 
Reconstitution:Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
 
Use/Stability:The stock solution should be stored in aliquotes at -20°C for 1 year or should be stored at +4°C for 6 months after adding 0.1% sodium azide.
 
Handling:Dilute only prior to immediate use. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Ca2+ should be contained in all the buffers to stabilize cadherin antigen.
 

Product Literature References

Transformation of cell adhesion properties by exogenously introduced E- cadherin cDNA: A. Nagafuchi, et al.; Nature 329, 341 (1987), Abstract;
Molecular nature of the calcium-dependent cell-cell adhesion system in mouse teratocarcinoma and embryonic cells studied with a monoclonal antibody: C. Yoshida-Noro, et al.; Dev. Biol. 101, 19 (1984), Abstract;

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