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β-Catenin monoclonal antibody (8E4)

 
ALX-804-260-C100 100 µg 443.00 USD
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Replaces Prod. #: ALX-804-260/1

Product Specification

Clone:8E4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to human β-catenin aa 27-37 conjugated to KLH.
 
UniProt ID:P35222
 
Species reactivity:Human, Mouse
Dog
 
Specificity:Recognizes an epitope between aa 27-37 and an additional epitope in the C-Terminal region.
 
Crossreactivity:Weakly cross-reacts with rat β-catenin.
 
Applications:ELISA, ICC, IHC (PS), WB
 
Recommended Dilutions/Conditions:ELISA (0.05µg/ml)
Immunocytochemistry (1-10µg/ml)
Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride., BSA may be used alternatively but milk is not recommended for blocking and dilution!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:For specific detection of dephospho-β-catenin use Prod. No. ALX-804-259.
 
Positive Control:Included. (Prod. No. ALX-840-022)
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
804-259 060 260 058
Figure A: Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies.
Figure B:
Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7)  (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)
804-260-1
Figure 2: Detection of endogenous β-Catenin.Whole cell lysates of serum starved tumor cells (20’000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with MAb to β-Catenin (8E4) (Prod. No. ALX-804-260/1) (0.5 µg/ml) for 1h at RT and developed by ECL (exp. time: 30s). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D
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804-259 060 260 058 804-260-1

Product Literature References

An aberrant nuclear localization of E-cadherin is a potent inhibitor of Wnt/β-catenin-elicited promotion of the cancer stem cell phenotype: Y. J. Su, et al.; Oncogenesis 4, e157 (2015), Application(s): Western Blot, Abstract; Full Text
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools: M. van Noort, et al.; Blood 110, 2778 (2007), Abstract;
Illegitimate WNT signaling promotes proliferation of multiple myeloma cells: P.W. Derksen, et al.; PNAS 101, 6122 (2004), Abstract;
Sulindac targets nuclear beta-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines: E.M. Boon, et al.; Br. J. Cancer 90, 224 (2004), Abstract;
Activation of the canonical beta-catenin pathway by histamine: S.H. Diks, et al.; J. Biol. Chem. 278, 52491 (2003), Abstract;
Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids: E.M. Schmelz, et al.; Cancer Res. 61, 6723 (2001), Abstract;

Related Products

β-Catenin monoclonal antibody (dephospho, aa 35-50) (7A7) 

ELISA, ICC, IHC (PS), IP, WB | Print as PDF
 
ALX-804-259-C100 100 µg 430.00 USD
Do you need bulk/larger quantities?
 

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