β-Catenin, mAb (dephospho, aa 35-50) (7A7)



ALX-804-259-C100	100 µg	379.00 USD

Product Specification

Purity Detail:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Formulation:	Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.

Clone:	7A7

Isotype:	Mouse IgG1

Immunogen:	Synthetic peptide corresponding to aa 35-50 of human β-catenin.

Reconstitution:	Reconstitute with 1ml distilled water (15 min., RT).

Specificity:	Recognizes human, mouse, rat (weak) and dog cytoplasmic dephospho β-catenin.

Application:	Immunocytochemistry (1-10µg/ml) Immunohistochemistry (paraffin sections) Immunoprecipitation Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. BSA may be used alternatively but milk is not recommended for blocking and dilution!

Long Term Storage:	-20°C

Use/Stability:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.

Handling:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.

Positive Control:	Included (Prod. No. ALX-840-022).

Figure A: Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies.
Figure B: Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7) (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)

Please mouse over

Product Literature References

A Formylated Hexapeptide Ligand Mimics the Ability of Wnt-5a to Impair Migration of Human Breast Epithelial Cells: A. Säfholm et al.; J. Biol. Chem. 281, 2740 (2006), Full Text