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RANKL monoclonal antibody (12A380)

 
ALX-804-244-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:ODF, TRANCE, OPGL, TNFSF 11, CD254
 
Clone:12A380
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant mouse RANKL (aa 1-317).
 
UniProt ID:O35235
 
Species reactivity:Human, Mouse
 
Applications:Flow Cytometry, IHC (FS), WB
 
Recommended Dilutions/Conditions:Western Blot (4-6µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~40kDa by Western blot.
 
Positive Control:Activated EL4 cells (mouse) or Saos-2 (human) cells can be used as positive control.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
 
Use/Stability:Stable for at least 6 months when stored at +4°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:RANKL (TRANCE, OPGL, ODF) is a member of the TNF family. RANKL (receptor activator of NF-κB ligand) or TRANCE (TNF-related activation-induced cytokine receptor) has been implicated in interactions between T cells and dendritic cells. RANKL binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. RANKL is also important in T- and B-cell maturation. As OPGL (osteoprotegerin ligand) or ODF (osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. RANKL deficient mice show severe osteoporesis and complete abscence of osteoclasts as a result of lack of osteogenesis.
 

Product Literature References

Expression of bone-regulatory proteins in human valve allografts: R. Shetty, et al.; Heart 92, 1303 (2006), Abstract; Full Text
Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis: T. Yoneda, et al.; Endocrinology 145, 2384 (2004), Abstract; Full Text
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001), Abstract; Full Text

General Literature References

OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis: Y.Y. Kong, et al.; Nature 397, 315 (1999), Abstract;
Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand: H. Hsu, et al.; PNAS 96, 3540 (1999), Abstract; Full Text
Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation: D.L. Lacey, et al.; Cell 93, 165 (1998), Abstract;
A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function: D.M. Anderson, et al.; Nature 390, 175 (1997), Abstract;
TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor: B.R. Wong, et al.; J. Exp. Med. 186, 2075 (1997), Abstract; Full Text

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