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Caspase-8 (human) monoclonal antibody (12F5)

ALX-804-242-C100 100 µg 401.00 USD
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Product Specification

Alternative Name:FLICE
Immunogen:Recombinant human caspase-8.
UniProt ID: Q14790
Species reactivity:Human
Specificity:Recognizes procaspase-8 and active caspase-8. Detects procaspase-8 (p55/54), the intermediate cleavage products (p43/41) and the p18 active subunit of caspase-8 by Western blot.
Crossreactivity:Does not cross-react with mouse caspase-8.
Applications:IHC (FS), IP, WB
Recommended Dilutions/Conditions:Western Blot (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In 0.15M PBS, pH 7.2, containing 1% BSA and 0.01% thimerosal.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
(A) Schematic structure of caspase-8 and its cleavage products as detected by anti-caspase-8 (12F5). The antibody is directed against the p18 subunit and detects procaspase-8 (p55/54), the intermediate cleavage products of 43kD and 41kD and the p18 active subunit of caspase-8.
(B) Detection of Fas ligand-induced caspase-8 processing and activation in human Jurkat cells.
Method: Jurkat cells were treated with Fas ligand (100ng/ml). After the indicated time points cell lysates were prepared and separated on a 12.5% SDS-PAGE (2x106 cells/lane) under reducing conditions. Proteins were immunoblotted with anti-caspase-8 12F5 (1µg/ml). Following incubation with peroxidase-conjugated secondary antibodies caspase-8 processing was detected by enhanced chemoluminescent staining. The open arrowhead indicates the two isoforms of procaspase-8 (caspase-8/a and caspase-8/b), which are cleaved into the intermediate forms p43 and p41 and finally processed to the active p18 subunit (filled arrowheads).
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Product Literature References

Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination: M.C. de Almagro, et al.; Cell Death Dis. 6, e1800 (2015), Application(s): Immunoprecipitation, Abstract; Full Text
Intestinal genetic inactivation of caspase-8 diminishes migration of enterocytes: E. Kaemmerer, et al.; World J Gastroenterol 21, 4499 (2015), Application(s): Western Blot, Abstract; Full Text
Up-regulation of Bcl-2 during adipogenesis mediates apoptosis resistance in human adipocytes: S.A. Nagel, et al.; Mol. Cell Endocrinol. 382, 368 (2014), Application(s): WB using human cell lysates, Abstract;
Prognostic and therapeutic relevance of FLIP and procaspase-8 overexpression in non-small cell lung cancer: J.S. Riley, et al.; Cell Death Dis. 4, e951 (2013), Application(s): WB using human cell lysates, Abstract; Full Text
Cathepsin D primes caspase-8 activation by multiple intra-chain proteolysis: S. Conus, et al.; J. Biol. Chem. 287, 21142 (2012), Application(s): WB using human cell lysates, Abstract; Full Text
Vorinostat/SAHA-induced apoptosis in malignant mesothelioma is FLIP/caspase 8-dependent and HR23B-independent: J.L. Hurwitz, et al.; Eur. J. Cancer 48, 1096 (2012), Application(s): WB using human cell and tissue lysates, Abstract;
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): WB using human neutrophil lysates, Abstract; Full Text
GDP-mannose-4,6-dehydratase (GMDS) deficiency renders colon cancer cells resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor- and CD95-mediated apoptosis by inhibiting complex II formation: K. Moriwaki, et al.; J. Biol. Chem. 286, 43123 (2011), Application(s): WB using human cell lysates, Abstract; Full Text
Endogenous Bak inhibitors Mcl-1 and Bcl-xL: differential impact on TRAIL resistance in Bax-deficient carcinoma: B. Gillissen, et al.; J. Cell Biol. 188, 851 (2010), Abstract;
A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-α-induced Apoptosis: V. Oliveira, et al.; J. Biol. Chem. 283, 13174 (2008), Abstract;
The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008), Abstract;
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP: J.J. Goval, et al.; Haematologica 93, 1169 (2008), Application(s): WB using human follicular lymphoma lysate, Abstract; Full Text
Constitutive caspase activation and impaired death-inducing signaling complex formation in CD95-resistant, long-term activated, antigen-specific T cells: G. Strauss, et al.; J. Immunol. 171, 1172 (2003), Abstract; Full Text
An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis: Y. Kim, et al.; J. Biol. Chem. 277, 22320 (2002), Abstract; Full Text
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway: D. Siegmund, et al.; J. Biol. Chem. 276, 32585 (2001), Abstract; Full Text
Trail-induced apoptosis in Type I leukemic cells is not enhanced by overexpression of bax: L. Jia, et al.; BBRC 283, 1037 (2001), Abstract;
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol. 30, 1769 (2000), Abstract;
Inhibition of death receptor-mediated gene induction by a cycloheximide-sensitive factor occurs at the level of or upstream of Fas-associated death domain protein (FADD): H. Wajant, et al.; J. Biol. Chem. 275, 24357 (2000), Abstract; Full Text
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood 93, 3053 (1999), Abstract; Full Text
P2Z purinoreceptor ligation induces activation of caspases with distinct roles in apoptotic and necrotic alterations of cell death: D. Ferrari, et al.; FEBS Lett. 447, 71 (1999), Abstract;
Sendai virus infection induces apoptosis through activation of caspase-8 (FLICE) and caspase-3 (CPP32): M. Bitzer, et al.; J. Virol. 73, 702 (1999), Abstract; Full Text
Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med. 188, 979 (1998), Abstract; Full Text

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