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[pSer239]VASP monoclonal antibody (16C2) (biotin conjugate)

 
ALX-804-240B-C100 100 µg Biotin 701.00 USD
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Product Specification

Alternative Name:Vasodilator stimulated phosphoprotein
 
Clone:16C2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE) conjugated to KLH.
 
UniProt ID:P50552
 
Species reactivity:Human, Mouse, Rat
Rabbit
 
Specificity:Recognizes Ser239-phosphorylated VASP.
 
Crossreactivity:Does not cross-react with the non-phosphorylated form of VASP.
 
Applications:ELISA, Flow Cytometry, ICC, IP, WB
 
Recommended Dilutions/Conditions:ELISA (use at 0.05µg/ml)
Immunocytochemistry (use at 1-10µg/ml; may tolerate 0.5% formaldehyde fixation)
Immunoprecipitation (use 1-10 µg per 106 pervanadate-treated A431 cells)
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Liquid. 0.5mg/ml in 2x PBS containing 0.09% sodium azide, PEG and sucrose.
 
Use/Stability:Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Important! The use of MAb to VASP (activated) (Ser239) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!
 

Product Literature References

Cyclic nucleotides modulate store-mediated calcium entry through the activation of protein-tyrosine phosphatases and altered actin polymerization in human platelets: J.A. Rosado, et al.; J. Biol. Chem. 276, 15666 (2001), Abstract; Full Text
Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Simulated Phosphoprotein in Aorta: A. Mülsch, et al.; Circulation 103, 2188 (2001), Abstract; Full Text
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem. 275, 33536 (2000), Abstract; Full Text
Flow cytometry analysis of intracell. VASP phosphorylation for the assessment of activating and inhibitory signal transduct. pathways in human platelets-definition & detect. of ticlopidine/clopidogrel: U.R. Schwarz, et al.; Thromb. Haemost. 82, 1145 (1999), Abstract;
Analysis and Regulation of Vasodilator-stimulated Phosphoprotein Serine 239 Phosphorylation in Vitro and in Intact Cells Using a Phosphospecific Monoclonal Antibody: A. Smolenski, et al.; J. Biol. Chem. 273, 20029 (1998), Abstract; Full Text

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