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[pSer239]VASP monoclonal antibody (16C2)

 
ALX-804-240-C100 100 µg Purified 615.00 USD
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Product Specification

Alternative Name:Vasodilator stimulated phosphoprotein
 
Clone:16C2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE) conjugated to KLH.
 
UniProt ID:P50552
 
Species reactivity:Human, Mouse, Rat
Rabbit
 
Specificity:Recognizes Ser239-phosphorylated VASP.
 
Crossreactivity:Does not cross-react with the non-phosphorylated form of VASP.
 
Applications:ELISA, Flow Cytometry, ICC, IP, WB
 
Recommended Dilutions/Conditions:ELISA (use at 0.05µg/ml)
Immunocytochemistry (use at 1-10µg/ml; may tolerate 0.5% formaldehyde fixation) , Immunoprecipitation (use 1-10 µg per 106 pervanadate-treated A431 cells)
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Important! The use of MAb to VASP (activated) (Ser239) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!
 
804-240
Figure: Western blot analysis to illustrate phosphospecificity.Whole cell extracts of control (co) or Forskolin (F) treated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca. 20’000 cells per lane) and transfereed to PVDF membranes. Immunoblots were probed with MAb to VASP (phosphorylated) (pSer239) (16C2) (Prod. No. ALX-804-240) for 1h at RT and developed by ECL (exp. time: 30 s).
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804-240

Product Literature References

Dual roles of quercetin in platelets: phosphoinositide-3-kinase and MAP kinases inhibition, and cAMP-dependent vasodilator-stimulated phosphoprotein stimulation: W.J. Oh, et al.; Evid. Based Complement Alternat. Med. 2012, 485262 (2012), Application(s): Western blot, Abstract; Full Text
Acyl derivatives of coenzyme A inhibit platelet function via antagonism at P2Y1 and P2Y12 receptors: a new finding that may influence the design of anti-thrombotic agents: P. Manolopoulos, et al.; Platelets 19, 134 (2008), Abstract; Full Text
The active metabolite of prasugrel effectively blocks the platelet P2Y12 receptor and inhibits procoagulant and pro-inflammatory platelet responses: H.M. Judge, et al.; Platelets 19, 125 (2008), Abstract;
Dual actions of dephostatin on the nitric oxide/cGMP-signalling pathway in porcine iliac arteries: A.A. Persson, et al.; Eur. J. Pharmacol. 521, 124 (2005), Application(s): Western blot, Abstract;
Characterisation of GEA 3175 on human platelets;: comparison with S-nitroso-N-acetyl-D, L-penicillamine: A.K. Asplund Persson, et al.; Eur. J. Pharmacol. 496, 1 (2004), Application(s): Western blot, Abstract;
The activity of constitutive nitric oxide synthase is increased by the pathway cAMP/cAMP-activated protein kinase in human platelets. New insights into the antiaggregating effects of cAMP-elevating agents.: I. Russo, et al.; Thromb. Res. 114, 265 (2004), Application(s): Western blot, Abstract;
Cyclic nucleotides modulate store-mediated calcium entry through the activation of protein-tyrosine phosphatases and altered actin polymerization in human platelets: J.A. Rosado, et al.; J. Biol. Chem. 276, 15666 (2001), Abstract; Full Text
Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Simulated Phosphoprotein in Aorta: A. Mülsch, et al.; Circulation 103, 2188 (2001), Abstract; Full Text
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem. 275, 33536 (2000), Abstract; Full Text
Flow cytometry analysis of intracell. VASP phosphorylation for the assessment of activating and inhibitory signal transduct. pathways in human platelets-definition & detect. of ticlopidine/clopidogrel: U.R. Schwarz, et al.; Thromb. Haemost. 82, 1145 (1999), Abstract;
Analysis and Regulation of Vasodilator-stimulated Phosphoprotein Serine 239 Phosphorylation in Vitro and in Intact Cells Using a Phosphospecific Monoclonal Antibody: A. Smolenski, et al.; J. Biol. Chem. 273, 20029 (1998), Abstract; Full Text

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