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Dnmt3b monoclonal antibody (52A1018)

ALX-804-233-C100 100 µg 334.00 USD
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Product Specification

Alternative Name:DNA methyltransferase-3b
Immunogen:Recombinant mouse Dnmt3b (DNA methyltransferase-3b).
UniProt ID:O88509
Species reactivity:Human, Mouse
Crossreactivity:Does not cross-react with Dnmt3a.
Applications:ICC, IP, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1-2µg/ml)
Immunoprecipitation (1-2µg/ml)
Western Blot (2-4µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~100kDa by Western blot.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
Use/Stability:Stable for 6 months when stored at +4°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:DNA methylation is an important regulator of genetic information, shutting down gene expression through the addition of methyl groups to cytosine (C) bases in the DNA. The establishment of DNA methylation patterns requires de novo methylation that occurs predominantly during early development and gametogenesis. DNA methyltransferase-1 (Dnmt1) "maintains" C methylation after DNA replication by methylating the daughter DNA strand using the methylation pattern of the parental strand as a template. Dnmt3a and Dnmt3b are essential for the de novo methylation. They show nonoverlapping functions in development, with Dnmt3b specifically required for methylation of centromeric minor satellite repeats. Both Dnmt3a and Dnmt3b expression is elevated in several tumor cell lines comparable to Dnmt1. It has also been suggested that mutations of Dnmt3b are responsible for the ICF immunodeficiency syndrome.
Figure: Western blot analysis of Dnmt3b in NIH 3T3 cell lysate using MAb to Dnmt3b (52A1018) (Prod. No. ALX-804-233) at 4µg/ml.
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Product Literature References

An association of DNMT3b protein expression with P16INK4a promoter hypermethylation in non-smoking female lung cancer with human papillomavirus infection: T.S. Lin, et al.; Cancer Lett. 226, 77 (2005), Abstract;
Reduced genomic cytosine methylation and defective cellular differentiation in embryonic stem cells lacking CpG binding protein: D.L. Carlone, et al.; Mol. Cell. Biol. 25, 4881 (2005), Abstract; Full Text
Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development: J.L. MacDonald, et al.; Dev. Biol. 288, 461 (2005), Abstract;
Induction of DNA methylation and gene silencing by short interfering RNAs in human cells: H. Kawasaki and K. Taira; Nature 431, 211 (2004), Abstract;
Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res. 64, 8184 (2004), Abstract;
Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation: D.J. Weisenberger, et al.; Mol. Cancer Res. 2, 62 (2004), Abstract; Full Text
Double RNA interference of DNMT3b and DNMT1 enhances DNA demethylation and gene reactivation: Y.W. Leu, et al.; Cancer Res. 63, 6110 (2003), Abstract;
The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription: R. Santoro, et al.; Nat. Genet. 32, 393 (2002), Abstract;

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