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FasL (human) monoclonal antibody (2C101)

 
ALX-804-232-C100 100 µg 452.00 USD
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Product Specification

Alternative Name:APO-1L, CD178, CD95L, TNFSF 6
 
Clone:2C101
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human FasL (CD95L; APO-1L; CD178) (extracellular domain).
 
UniProt ID:P48023
 
Species reactivity:Human
 
Applications:Flow Cytometry, ICC, FUNC
 
Recommended Dilutions/Conditions:Suggested starting dilution is >1:100. , Optimal conditions must be determined individually for each application.
 
Application Notes:Excellent for Functional Application (neutralizing).
 
Purity Detail:Affinity purified.
 
Formulation:Liquid. In 0.15M PBS, pH 7.2, containing 1% BSA. Contains no preservatives.
 
Use/Stability:Stable for at least 6 months when stored at +4°C.
 
Handling:Keep sterile. Do not freeze.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
FasL (human) monoclonal antibody (2C101) Schematic structure of immunogen
Figure: Schematic structure of recombinant human FasL (extracellular domain).
FasL (human) monoclonal antibody (2C101) Flow Cytometry
Figure: Flow cytometric detection of FasL expression in stimulated H9 T-cells.Method: H9 T-cells (1x106) were either left untreated (open histogram) or stimulated with 10ng/ml PMA and 1µg/ml ionomycin (filled histogram). After 12 hours cells were harvested and stained with anti-FasL 2C101 (1µg/ml), followed by biotinylated goat anti-mouse IgG and streptavidin-PE.
FasL (human) monoclonal antibody (2C101) Flow Cytometry
Figure: Inhibition of apoptosis in anti-CD3 stimulated Jurkat T-cells by anti-Fas L.Method: Jurkat JR cells (2x106) were stimulated with plate-bound anti-CD3 antibodies (10µg/ml) in the presence or absence of the indicated concentrations of anti-FasL 2C101 or a control IgG1 mouse monoclonal antibody. After 48 hours apoptosis was evaluated by flow cytometric analysis of PI-stained nuclei.
FasL (human) monoclonal antibody (2C101) Flow Cytometry
Figures: Anti-FasL MAb ALX-804-232 on HEK 293 wt. and HEK 293-FasL clone cells expressing FasL fusion protein. HEK 293 cells transfected with membrane bound FasL (right figure) were compared with HEK 293 untransfected control cells (left figure).  "
FasL (human) monoclonal antibody (2C101) Flow Cytometry
Figure: Comparison of anti-FasL MAbs 804-231 and 804-232 on 293-FasL clone cells expressing FasL fusion protein. Comparable results were obtained for all other MAbs to hFasL recommended for flow cytometry.
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FasL (human) monoclonal antibody (2C101) Schematic structure of immunogen FasL (human) monoclonal antibody (2C101) Flow Cytometry FasL (human) monoclonal antibody (2C101) Flow Cytometry FasL (human) monoclonal antibody (2C101) Flow Cytometry FasL (human) monoclonal antibody (2C101) Flow Cytometry

Product Literature References

IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt et al.; Eur. J. Immunol. 30, 1769 (2000), Abstract;

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