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FasL (human) monoclonal antibody (2C101)

ALX-804-232-C100 100 µg 452.00 USD
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Product Specification

Alternative Name:APO-1L, CD178, CD95L, TNFSF 6
Immunogen:Recombinant human FasL (CD95L; APO-1L; CD178) (extracellular domain).
UniProt ID:P48023
Species reactivity:Human
Applications:Flow Cytometry, ICC, FUNC
Recommended Dilutions/Conditions:Suggested starting dilution is >1:100. , Optimal conditions must be determined individually for each application.
Application Notes:Excellent for Functional Application (neutralizing).
Purity Detail:Affinity purified.
Formulation:Liquid. In 0.15M PBS, pH 7.2, containing 1% BSA. Contains no preservatives.
Use/Stability:Stable for at least 6 months when stored at +4°C.
Handling:Keep sterile. Do not freeze.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Figure: Schematic structure of recombinant human FasL (extracellular domain).
Figure: Flow cytometric detection of FasL expression in stimulated H9 T-cells.Method: H9 T-cells (1x106) were either left untreated (open histogram) or stimulated with 10ng/ml PMA and 1µg/ml ionomycin (filled histogram). After 12 hours cells were harvested and stained with anti-FasL 2C101 (1µg/ml), followed by biotinylated goat anti-mouse IgG and streptavidin-PE.
Figure: Inhibition of apoptosis in anti-CD3 stimulated Jurkat T-cells by anti-Fas L.Method: Jurkat JR cells (2x106) were stimulated with plate-bound anti-CD3 antibodies (10µg/ml) in the presence or absence of the indicated concentrations of anti-FasL 2C101 or a control IgG1 mouse monoclonal antibody. After 48 hours apoptosis was evaluated by flow cytometric analysis of PI-stained nuclei.
Figures: Anti-FasL MAb ALX-804-232 on HEK 293 wt. and HEK 293-FasL clone cells expressing FasL fusion protein. HEK 293 cells transfected with membrane bound FasL (right figure) were compared with HEK 293 untransfected control cells (left figure).  
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Figure: Comparison of anti-FasL MAbs 804-231 and 804-232 on 293-FasL clone cells expressing FasL fusion protein. Comparable results were obtained for all other MAbs to hFasL recommended for flow cytometry.
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Product Literature References

IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt et al.; Eur. J. Immunol. 30, 1769 (2000), Abstract;

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