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Bax (active monomer) monoclonal antibody (6A7)

 
ALX-804-224-C100 100 µg 291.00 USD
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Product Specification

Alternative Name:BCL2L4, BCL2-associated X protein
 
Clone:6A7
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 12-24 of human Bax.
 
UniProt ID:Q07812
 
Species reactivity:Human, Mouse, Rat
 
Specificity:Recognizes the active monomeric form of Bax.
 
Crossreactivity:Does not cross-react with Bax complexed with either Bcl-2 or Bcl-XL or to inactive monomer.
 
Applications:ELISA, ICC, IP, WB
 
Recommended Dilutions/Conditions:ELISA (0.5-1µg/ml)
Immunocytochemistry (0.5-1µg/ml)
Immunoprecipitation (in detergent)
Western Blot (0.5-1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS.
 
Handling:After opening aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:Bax and Bak operate in both the endoplasmatic reticulum (ER) and mitochondria as an essential gateway for selected apoptotic signals. A role for Bcl-2 at the ER has been suggested earlier as Bcl-2 may regulate calcium fluxes between the ER and the mitochondria. For a review on Ca2+ and its role in apoptosis.
 

Product Literature References

Nitro-Oleic Acid Attenuates OGD/R-Triggered Apoptosis in Renal Tubular Cells via Inhibition of Bax Mitochondrial Translocation in a PPAR-γ-Dependent Manner: H. Nie, et al.; Cell Physiol. Biochem. 35, 1201 (2015), Application(s): Immunocytochemistry, Abstract; Full Text
Vinblastine-induced apoptosis is mediated by discrete alterations in subcellular location, oligomeric structure, and activation status of specific Bcl-2 family members: M. Upreti, et al.; J. Biol. Chem. 281, 15941 (2006), Abstract; Full Text
Interaction with a membrane surface triggers a reversible conformational change in Bax normally associated with induction of apoptosis: J.A. Yethon, et al.; J. Biol. Chem. 278, 48935 (2003), Abstract;
Direct addition of BimL to mitochondria does not lead to cytochrome c release: O. Terradillos, et al.; FEBS Lett. 522, 29 (2002), Abstract;
Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations: Y.T. Hsu and R.J. Youle; J. Biol. Chem. 273, 1077 (1998), Abstract; Full Text
Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis: Y.T. Hsu, et al.; PNAS 94, 3668 (1997), Abstract; Full Text
Nonionic detergents induce dimerization among members of the Bcl-2 family: Y.T. Hsu and R.J. Youle; J. Biol. Chem. 272, 13829 (1997), Abstract; Full Text

General Literature References

BAX and BAK regulation of endoplasmic reticulum Ca2+: a control point for apoptosis: L. Scorrano, et al.; Science 300, 135 (2003), Abstract;
Calcium signaling and apoptosis: G. Hajnoczky, et al.; BBRC 304, 445 (2003), Abstract;
New insights in the role of Bcl-2 Bcl-2 and the endoplasmic reticulum: J. Rudner, et al.; Apoptosis 7, 441 (2002), Abstract;

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