Product Specification
| Clone: | G8 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant v-Ski. |
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| UniProt ID: | P12755 |
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| Species reactivity: | Human, Mouse, Rat
Chicken, Xenopus
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| Specificity: | Recognizes cellular and hyperphosphorylated Ski. |
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| Applications: | ICC, IP, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1-10µg/ml)
Immunoprecipitation (1-4µg per sample)
Western Blot (1-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects bands of ~95kDa (Ski) and bands of up to ~115kDa (hyperphosphorylated Ski) by Western blot. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. It has been shown that Ski associates with Smads2, 3 and 4 proteins in response to TGF-β stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF-β-induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF-β family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation. |
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Figure: Western blot: Rat cells infected with (lane 1) pBABEpuro h-Ski expressing full length human Ski proteins of ~95kDa and 100kDa (hyperphosphorylated) and (lane 2) infected with pBABEpuro vector showing endogenous Ski proteins ranging from ~97 kDa to 115 kDa. Blot probed with MAb to Ski (G8) (Prod. No. ALX-804-221) at 1µg/ml.
Figure: Immunocytochemistry: Photomicrograph illustrating retrovirally over-expressed v-Ski infibroblasts stained with MAb to Ski (G8) (Prod. No. ALX-804-221).
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Product Literature References
Association of specific DNA binding and transcriptional repression with the transforming and myogenic activities of c-Ski: R. Nicol, et al.; Cell Growth Differ.
10, 243 (1999),
Abstract;
Full Text
Heterodimers of the SnoN and Ski oncoproteins form preferentially over homodimers and are more potent transforming agents: S.B. Cohen, et al.; Nucl. Acids Res.
27, 1006 (1999),
Abstract;
Full Text
General Literature References
c-Ski acts as a transcriptional Co-repressor in transforming growth factor-beta signaling through interaction with smads : S. Akiyoshi, et al. ; J. Biol. Chem.
274, 35269 (1999),
Abstract;
Full Text
Interaction of the Ski oncoprotein with Smad3 regulates TGF-beta signaling: Y. Sun, et al.; Mol. Cell
4, 499 (1999),
Abstract;
Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor : T. Nomura, et al.; Genes Dev.
13, 412 (1999),
Abstract;
Full Text
SnoN and Ski protooncoproteins are rapidly degraded in response to transforming growth factor beta signaling: Y. Sun, et al.; PNAS
96, 12442 (1999),
Abstract;
Full Text
The Ski oncoprotein interacts with the Smad proteins to repress TGFbeta signaling: K. Luo, et al.; Genes Dev.
13, 2196 (1999),
Abstract;
Full Text
Viral ski inhibits retinoblastoma protein (Rb)-mediated transcriptional repression in a dominant negative fashion : F. Tokitou, et al.; J. Biol. Chem.
274, 4485 (1999),
Abstract;
Full Text
The ski oncogene: P. Sutrave & S.H. Hughes; Oncogene
6, 353 (1991), (Review),
Abstract;
