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HIF-1α monoclonal antibody (mgc3)

 
ALX-804-216-R100 100 µl 619.00 USD
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Product Specification

Alternative Name:Hypoxia-inducible factor 1α, ARNT-interacting protein
 
Clone:mgc3
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Human HIF-1α (hypoxia-inducible factor 1α) (aa 530-826).
 
UniProt ID:Q16665
 
Species reactivity:Human, Mouse
Bovine, Porcine, Primate
 
Crossreactivity:Does not cross-react with HIF-1β or HIF-2α.
 
Applications:ICC, IP, WB
Gel Supershift
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:200)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~116kDa by Western blot after hypoxic induction.
Gel Supershift Assay: detects also mouse HIF-1α
 
Formulation:Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1α (HIF-1α) has been identified as a bHLH-PAS family member which is instrumental in the oxygen-dependent regulation of these genes. HIF-1α rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerizes with the aryl hydrocarbon nuclear receptor translocator, ARNT, also referred to as HIF-1β.
 
804-216
Figure A: Western blot using MAb to Hypoxia-inducible Factor 1α (mgc3) (Prod. No. ALX-804-216).Method: Nuclear extracts (25µg) from normoxic (20% O2) and hypoxic (1% O2) human HeLa and mouse Hepa1 cells were subjected to SDS-PAGE and HIF-1α was detected in Western blots using MAb mgc3 (1:1’000). Note that mgc3 detects denatured HIF-1α only in human cells in Western blot analysis [1].
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Figure B: Electrophoretic mobility shift assay (EMSA) using MAb to Hypoxia-inducible Factor 1α (mgc3) (Prod. No. ALX-804-216).Method: Nuclear extracts (5µg) from normoxic and hypoxic human HeLa and mouse wild-type (Hepa1) and ARNT-mutant (Hepa1C4) hepatoma cells were analyzed by EMSA. MAb mgc3 supershifted the HIF-1 heterodimer in HeLa and Hepa1 cells. No HIF DNA-binding activity was detected in ARNT-mutant Hepa1C4 cells. In contrast to Western blot analysis, mgc3 was able to detect also non-human HIF-1α in EMSA [1,2].
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Product Literature References

Preconditional activation of hypoxia-inducible factors ameliorates ischemic acute renal failure: W.M. Bernhardt, et al.; Am. J. Soc. Nephrol. 17, 1970 (2006), Abstract; Full Text
General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobuilins raised against thy hypoxia-inducible factor 1alpha: G. Camenisch, et al.; FASEB J. 13, 81 (1999), Abstract; Full Text
Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha: D. Chilov, et al.; J. Cell. Sci. 112, 1203 (1999), Abstract; Full Text

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