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NF-ATc2 monoclonal antibody (25A10.D6.D2)

 
ALX-804-215-C100 100 µg 594.00 USD
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Product Specification

Alternative Name:NFATc2, Nuclear factor of activated T cells, cytoplasmic 2, NFATP, NFAT1
 
Clone:25A10.D6.D2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 51-69 (A51ISSPSGLAYPDDVLDYGL69) of mouse nuclear factor of activated T cells, cytoplasmic 2 (NF-ATc2) conserved in all three isoforms A, B and C.
 
UniProt ID:Q60591
 
Species reactivity:Human, Mouse, Rat
 
Crossreactivity:Does not cross-react with NF-ATc1.
 
Applications:ICC, IF, IHC (FS), IHC (PS), IP, WB
Gel Supershift
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100 - 1:1,000)
Immunofluorescence (1:100 - 1:1,000)
Immunohistochemistry (frozen sections and paraffin sections, 1:50 - 1:200)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~140kDa (phosphorylated NF-ATc2 in resting immune cells) and ~120kDa (fully-dephosphorylated NF-ATc2 in stimulated cells) by Western blot.
 
Formulation:Liquid. Purified antibody in PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Note: Confusion may arise regarding NFAT protein versus gene names. According to UniProt, the nomenclature is as follows:Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells (NF-AT5)Gene Names: NFAT5
 

Product Literature References

The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements: H.P. Kim & W. Leonard; EMBO J. 21, 3051 (2002), Abstract; Full Text
Identification and characterization of a novel nuclear factor of activated T-cells-1 isoform expressed in mouse brain: S. Plyte, et al.; J. Biol. Chem. 276, 14350 (2001), Abstract; Full Text
The calcineurin-NFAT pathway and muscle fiber-type gene expression: S.J. Swoap, et al.; Am. J. Physiol. Cell Physiol. 279, C915 (2000), Abstract; Full Text

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