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Apurinic/apyrimidinic endonuclease (human) monoclonal antibody (13B 8E5C2)

 
ALX-804-213-R100 100 µl 583.00 USD
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Product Specification

Alternative Name:HAP1, APEX, APE1, Ref-1
 
Clone:13B 8E5C2
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
 
UniProt ID:P27695
 
Species reactivity:Human
 
Applications:IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~37kDa by Western blot.
 
Formulation:Liquid. In PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κ B, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5’ of an AP site to produce a hydroxyl group at the 3’ terminus of an unmodified nucleotide upstream of the nick and a 5’-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5’-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3’-5’ DNA exonuclease activity, a 3’-phosphodiesterase activity, a 3’-phosphatase activity, and a RNase H activity. It has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3’ termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.
 
804-213
Figure: Immunohistochemistry of APE/ref-1 in prostatic carcinoma using MAb to Apurinic/Apyrimidinic Endonuclease (human) (13B 8E5C2) (Prod. No. ALX-804-213).
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804-213

Product Literature References

Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancer: M.R. Kelley, et al.; Clin. Cancer Res. 7, 824 (2001), Abstract;
Alterations in the expression of the DNA repair/redox enzyme APE/ref-1 in epithelial ovarian cancers: D.H. Moore, et al.; Clin. Cancer Res. 6, 602 (2000), Abstract;

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