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PARP-1 monoclonal antibody (F1-23)

 
ALX-804-211-R050 50 µl 430.00 USD
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Replaces Prod. #: BML-SA251

Product Specification

Alternative Name:Poly(ADP-ribose) polymerase-1
 
Clone:F1-23
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Purified bovine PARP-1 (poly(ADP-ribose) polymerase-1).
 
UniProt ID:P18493
 
Species reactivity:Human
Bovine
 
Specificity:Recognizes an epitope in the zinc finger II DNA binding domain of PARP-1.
 
Crossreactivity:Does not cross-react with mouse or rat PARP-1.
 
Applications:ELISA, ICC, IP, WB, FUNC
ChIP
 
Recommended Dilutions/Conditions:ELISA: >1:200,000. Dilution buffer: PBS, pH 7.4, 1% non fat dried milk, 0.05% Tween 20. Detectable antigen range: 50ng of PARP-1
Immunocytochemistry: 1:500. Dilution buffer: 50mM TRIS, pH 7.5, 150mM NaCl, 5% milk.
Immunoprecipitation: 1:50. Dilution buffer: RiPA buffer Protein G-Sepharose™
Western Blot: 1:5,000. Dilution buffer: PBS, pH 7.4, 5% non fat dried milk, 0.1% Tween 20. See also separate Western blot protocol.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~116kDa (intact PARP-1) and ~24kDa (apoptosis-induced cleavage fragment) by Western blot.
Functional Application: neutralizes the biological activity of PARP (~80%).
 
Formulation:Liquid. Ascites fluid containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles. For long-term storage, aliquot into individual tubes and refreeze at -80°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-80°C
 
Technical Info/Product Notes:SEPHAROSE is a trademark of GE Healthcare companies.
 

Product Literature References

DNA-dependent SUMO modification of PARP-1: N. Zilio, et al.; DNA Repair (Amst) 12, 761 (2013), Application(s): Western blot, Abstract; Full Text
The histone variant mH2A1.1 interferes with transcription by down-regulating PARP-1 enzymatic activity: K. Ouararhni, et al.; Genes Dev. 20, 3324 (2006), Abstract; Full Text
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression: A. Augustin, et al.; J. Cell Sci. 116, 1551 (2003), Abstract;
Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase: P.J. Duriez, et al.; Biochim. Biophys. Acta 1334, 65 (1997), Abstract;
FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis: A.M. Chinnaiyan, et al.; J. Biol. Chem. 271, 4961 (1996), Abstract; Full Text
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995), Abstract; Full Text
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;
Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis: S.H. Kaufmann, et al.; Cancer Res. 53, 3976 (1993), Abstract;
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988), Abstract;

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