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E-cadherin (human) monoclonal antibody (SHE78-7)

 
ALX-804-203-C100 100 µg 690.00 USD
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Product Specification

Alternative Name:Uvomorulin, CD324, Epithelial cadherin, Cadherin-1
 
Clone:SHE78-7
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Soluble E-cadherin from human placenta.
 
UniProt ID:P12830
 
Source:Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with soluble E-cadherin from human placenta.
 
Species reactivity:Human
 
Crossreactivity:Does not cross-react with rat E-cadherin.
 
Applications:ELISA, Flow Cytometry, IF, IHC (FS), IHC (PS), WB, FUNC
 
Recommended Dilutions/Conditions:ELISA (2µg/ml)
Flow Cytometry (1µg/ml)
Immunofluorescence  (1µg/ml)
Immunohistochemistry (frozen sections, 2µg/ml)
Immunohistochemistry (paraffin sections, 2µg/ml; good results were obtained by incubating with the primary antibody at 4°C overnight)
Western Blot (2µg/ml; reducing and non-reducing conditions)
Functional Application (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Functional Application: inhibits of E-cadherin-dependent cell-cell contact
 
Purity Detail:Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography.
 
Formulation:Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Contains no preservatives.
 
Reconstitution:Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.Note: TBS should be used for dilution, as PBS causes precipitation.
 
Use/Stability: The stock solution should be stored in aliquotes at -20°C for 1 year or should be stored at 4°C for 6 months after adding 0.1% sodium azide.
 
Handling:Dilute only prior to immediate use. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Technical Info/Product Notes:Ca2+ should be contained in the buffers to stabilize cadherin antigen. In case of immunohistochemical staining of paraffin embedded tissues and acetone fixed frozen sections, Ca2+ is not necessary.
 

Product Literature References

Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersed carcinoma line: M. Watabe, et al.; J. Cell. Biol. 127, 247 (1994), Abstract; Full Text
Soluble E-cadherin fragments increased in circulation of cancer patients: M. Katayama, et al.; Br. J. Cancer 69, 580 (1994), Abstract;
Soluble fragment of E-cadherin cell adhesion molecule increase in urinary excretion of cancer patients, potentially indicating its shedding from epithelial tumour cells: M. Katayama, et al.; Int. J. Oncol. 5, 1049 (1994),

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