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E-cadherin monoclonal antibody (ECCD-2)

 
ALX-804-202-C100 100 µg 635.00 USD
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Product Specification

Alternative Name:Uvomorulin, CD324, Epithelial cadherin, Cadherin-1
 
Clone:ECCD-2
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Mouse liver E-cadherin fragment.
 
UniProt ID:P09803
 
Source:Obtained by fusing the P3-X63-Ag8 myeloma cell line with splenocytes of Donryu rat immunized with mouse liver E-cadherin fragment.
 
Species reactivity:Human, Mouse
Dog
 
Crossreactivity:Does not cross-react with bovine or chicken E-cadherin.
 
Applications:IHC (FS), WB, FUNC
 
Recommended Dilutions/Conditions:Immunohistochemistry (frozen sections, 10µg/ml)
Western Blot (10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Not recommended for Immunohistochemistry (paraffin sections).
Functional Application: Does not inhibit E-cadherin dependent cell-cell contact and can be used to analyze the mechanism.
 
Purity Detail:Purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography.
 
Formulation:Lyophilized from 20mM TBS, pH 7.5, containing 1% BSA. Contains no preservatives.
 
Reconstitution:Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
 
Use/Stability:Stable for at least 2 years after receipt when stored at +4°C. Reconstituted antibody is stable for 6 months when stored at +4°C and for 1 year when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Buffers should contain Ca2+ to stabilize cadherin antigen. In case of immunohistochemical staining of acetone fixed frozen sections, Ca2+ is not necessary.
 

Product Literature References

Inverse correlation between the extent of N-glycan branching and intercellular adhesion in epithelia. Contribution of the Na,K-ATPase beta1 subunit: O. Vagin, et al.; J. Biol. Chem. 283, 2192 (2008), Abstract; Full Text
Recruitment and activation of Rac1 by the formation of E-cadherin- mediated cell-cell adhesion sites: M. Nakagawa, et al.; J. Cell. Sci. 114, 1829 (2001), Abstract;
The alpha isoform of protein kinase C is involved in signaling the response of desmosomes to wounding in cultured epithelial cells: S. Wallis, et al.; Mol. Biol. Cell. 11, 1077 (2000), Abstract;
Role of IQGAP1, a target of the small GTPase Cdc42 and Rac1, in regulation of E-cadherin-mediated cell-cell adhesion: S. Kuroda, et al.; Science 281, 832 (1998), Abstract;
Recognition of E-cadherin on epithelial cells by the mucosal T cell integrin alpha M290 beta 7 (alpha E beta 7): P.I. Karecla, et al.; Eur. J. Immunol. 25, 852 (1995), Abstract;
Cadherin cell adhesion receptors as a morphogenetic regulator: M. Takeichi; Science 251, 1451 (1991), Abstract;
The cadherins: cell-cell adhesion molecules controlling animal morphogenesis: M. Takeichi; Development 102, 639 (1988), Abstract;
Transformation of cell adhesion properties by exogenously introduced E- cadherin cDNA: A. Nagafuchi, et al.; Nature 329, 341 (1987), Abstract;
N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin: Y. Shirayoshi, et al.; Cell Struct. Funct. 11, 245 (1986), Abstract;

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