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E-cadherin monoclonal antibody (HECD-1)

 
ALX-804-201-C100 100 µg 690.00 USD
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Product Specification

Alternative Name:Uvomorulin, CD324, Epithelial cadherin, Cadherin-1
 
Clone:HECD-1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Human breast tumor cell line MCF-7.
 
UniProt ID:P12830
 
Source:Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with human breast tumor cell line MCF-7.
 
Species reactivity:Human
Guinea pig
 
Crossreactivity:Does not cross-react with rat, dog, bovine or chicken E-cadherin.
 
Applications:ELISA, Flow Cytometry, ICC, IHC (FS), IHC (PS), WB, FUNC
 
Recommended Dilutions/Conditions:ELISA (2-10µg/ml)
Flow Cytometry (1µg/ml)
Immunocytochemistry (1µg/ml)
Immunohistochemistry (frozen sections (2-10µg/ml)
paraffin sections (2-10µg/ml))
Western Blot (2-10µg/ml)
Functional Application (100µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Functional Application: inhibits of E-cadherin dependent cell-cell contact.
 
Purity Detail:Ammonium sulfate precipitation and ion exchange chromatography.
 
Formulation:Lyophilized from 10mM PBS, pH 7.4 , containing 1% BSA. Contains no preservatives.
 
Reconstitution:Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
 
Use/Stability:The stock solution should be stored in aliquotes at -20°C for 1 year or should be stored at 4°C for 6 months after adding 0.1% sodium azide.
 
Handling:Dilute only prior to immediate use. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:Ca2+ should be contained in all buffers to stabilize cadherin antigen.
 

Product Literature References

Changing etiology of tubal pregnancy following IVF: A. Revel, et al.; Hum. Reprod. 6, 1372 (2008), Abstract;
CD123bright plasmacytoid predendritic cells: progenitors undergoing cell fate conversion?: M.R. Comeau, et al.; J. Immunol. 169, 75 (2002), Abstract; Full Text
Differential expression of E-cadherin in normal, metaplastic and dysplastic esophageal mucosa - a putative biomarker: J.A. Jankowski, et al.; Int. J. Oncol. 4, 441 (1994), Abstract;
Immunohistochemical localization of cell adhesion molecule epithelial cadherin in human arachnoid villi and meningiomas: Y. Tohma, et al.; Cancer Res. 52, 1981 (1992), Abstract;
Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections: S.R. Shi, et al.; J. Histochem. Cytochem. 39, 741 (1991), Abstract;
Cadherin cell adhesion receptors as a morphogenetic regulator: M. Takeichi; Science 251, 1451 (1991), Abstract;
Cadherin intercellular adhesion molecule in hepatocellular carcinomas: loss of E-cadherin expression in an undifferentiated carcinoma: Y. Shimoyama & S. Hirohashi; Cancer Lett. 57, 131 (1991), Abstract;
Expression of E- and P-cadherin in gastric carcinomas: Y. Shimoyama & S. Hirohashi; Cancer Res. 51, 2185 (1991), Abstract;
Expression of immunoreactive E-cadherin adhesion molecules in human cancers: H. Shiozaki, et al.; Am. J. Pathol. 139, 17 (1991), Abstract;
Cadherin cell-adhesion molecules in human epithelial tissues and carcinomas: Y. Shimoyama, et al.; Cancer Res. 49, 2128 (1989), Abstract;

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