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TNF-α (human) monoclonal antibody (TNF-D)

 
ALX-804-199-C100 100 µg 269.00 USD
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Product Specification

Alternative Name:TNFSF 2, Tumor necrosis factor-α
 
Clone:TNF-D
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human TNF-α (tumor necrosis factor-α).
 
UniProt ID:P01375
 
Species reactivity:Human
 
Crossreactivity:Does not cross-react with mouse TNF-α or human or mouse TNF-β.
 
Applications:ELISA, Flow Cytometry, FUNC
 
Recommended Dilutions/Conditions:ELISA (use as capture or detection antibody in sandwich ELISA)
Flow Cytometry (suitable as primary antibody for staining in FACS analysis; recommended concentration is 10µg/ml as determined on PBMC. Method: PBMC were incubated with PMA, ionomycin, and with monensin to inhibit secretion. Incubation for 20 minutes with TNF-D at 10µg/ml; stained with goat-anti-mouse IgG-FITC second step reagent for 15 minutes)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Functional Application: selectively neutralizes human TNF-α bioactivity. TNF-D requires an incubation of antigen and antibody for 1 hour at 37 °C or 16 hours at 4 °C to completely neutralize TNF-α cytolytic activity.
Not recommended for Western blot.
 
Formulation:Liquid. In PBS.
 
Handling:After opening aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 

Product Literature References

Monoclonal antibodies to human tumor necrosis factors alpha and beta: application for affinity purification, immunoassays, and as structural probes: T.S. Bringman & B.B. Aggarwal; Hybridoma 6, 489 (1987), Abstract;

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