| ALX-804-199-C100 | 100 µg | 372.00 USD |  |
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| Alternative Name: | TNFSF 2, Tumor necrosis factor-α |
| Clone: | TNF-D |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human TNF-α (tumor necrosis factor-α). |
| UniProt ID: | P01375 |
| Species reactivity: | Human |
| Crossreactivity: | Does not cross-react with mouse TNF-α or human or mouse TNF-β. |
| Applications: | ELISA, Flow Cytometry, FUNC |
| Recommended Dilutions/Conditions: | ELISA (use as capture or detection antibody in sandwich ELISA) Flow Cytometry (suitable as primary antibody for staining in FACS analysis; recommended concentration is 10µg/ml as determined on PBMC. Method: PBMC were incubated with PMA, ionomycin, and with monensin to inhibit secretion. Incubation for 20 minutes with TNF-D at 10µg/ml; stained with goat-anti-mouse IgG-FITC second step reagent for 15 minutes) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Functional Application: selectively neutralizes human TNF-α bioactivity. TNF-D requires an incubation of antigen and antibody for 1 hour at 37 °C or 16 hours at 4 °C to completely neutralize TNF-α cytolytic activity. Not recommended for Western blot. |
| Formulation: | Liquid. In PBS. |
| Handling: | After opening aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles. |
| Shipping: | Blue Ice |
| Long Term Storage: | +4°C |
| Regulatory Status: | RUO - Research Use Only |
