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Phosphotyrosine monoclonal antibody (16F4)

 
ALX-804-174-C100 100 µg 323.00 USD
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Product Specification

Clone:16F4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Phosphotyrosine containing peptides conjugated to KLH.
 
Species reactivity:Species independent
 
Specificity:Epitope: ...DIpYAE...Recognizes phosphotyrosine in the context of the surrounding amino acids, tolerating hydrophobic amino acids directly neighbouring the phosphotyrosine.
 
Applications:ELISA, IP, WB
 
Recommended Dilutions/Conditions:Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Included. (Prod. No. 850-051-KI02)
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

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