| Clone: | 16F4 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Phosphotyrosine containing peptides conjugated to KLH. |
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| Species reactivity: | Species independent
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| Specificity: | Epitope: ...DIpYAE...Recognizes phosphotyrosine in the context of the surrounding amino acids, tolerating hydrophobic amino acids directly neighbouring the phosphotyrosine. |
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| Applications: | ELISA, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Positive Control: | Included. (Prod. No. 850-051-KI02) |
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| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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