Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Phosphothreonine monoclonal antibody (1E11)

 
ALX-804-168-C100 100 µg 536.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-SA338

Product Specification

Clone:1E11
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Phosphothreonine containing peptides conjugated to KLH.
 
Species reactivity:Species independent
 
Specificity:Recognizes a broad range of threonine-phosphorylated proteins.
 
Applications:ELISA, IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Included. (Prod. No. 840-012-1)
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 1 week.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Replacement for ADI-905-808
 

Product Literature References

Regulation of the ligand binding activity of the human very low density lipoprotein receptor by protein kinase C-dependent phosphorylation: R. Sakthivel, et al.; J. Biol. Chem. 276, 555 (2001), Abstract; Full Text
Insulin-mimetic signalling of synthetic phosphoinositolglycans in isolated rat adipocytes: W. Frick, et al.; Biochem. J. 336 (Pt 1), 163 (1998), Abstract; Full Text

Related Products

Phosphothreonine detection set 

IP, WB | Print as PDF
 
ALX-850-024-KI01 1 Set 432.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Phosphoprotein
By biological activity:
Phosphoprotein Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,