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Phosphoserine monoclonal antibody (16B4)

 
ALX-804-167-C100 100 µg 552.00 USD
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Replaces Prod. #: BML-SA337

Product Specification

Clone:16B4
 
Host:Mouse
 
Isotype:IgM
 
Immunogen:Phosphoserine containing peptides conjugated to KLH.
 
Species reactivity:Species independent
 
Specificity:Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, strongly interacting with pSK and pSP motifs, thus specifically recognizing substrates of MAP/SAP kinases and CDC kinases. Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
 
Applications:ELISA, IP, WB
 
Recommended Dilutions/Conditions:ELISA (use at 0.05µg/ml)
Western Blot (1µg/ml HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Included. (Prod. No. 840-012-1)
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 1 week.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Replacement for ADI-905-805
 

Product Literature References

Protein phosphatase 2A regulates apoptosis in neutrophils by dephosphorylating both p38 MAPK and its substrate caspase 3: M. Alvarado-Kristensson and T. Andersson; J. Biol. Chem. 280, 6238 (2005), Abstract; Full Text
p38-MAPK signals survival by phosphorylation of caspase-8 and caspase-3 in human neutrophils: M. Alvarado-Kristensson, et al.; J. Exp. Med. 199, 449 (2004), Abstract; Full Text
Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils: S.J. Gardai, et al.; J. Biol. Chem. 279, 21085 (2004), Abstract; Full Text
Kennedy’s disease. Phosphorylation of the polyglutamine-expanded form of androgen receptor regulates its cleavage by caspase-3 and enhances cell death: M.A. LaFevre-Bernt and L.M. Ellerby; J. Biol. Chem. 278, 34918 (2003), Abstract; Full Text
Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt, et al.; Proteomics 3, 1637 (2003), Abstract;
Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway: E.J. Tisdale; J. Biol. Chem. 277, 3334 (2002), Abstract; Full Text
Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites: S. Suer, et al.; Eur. J. Biochem. 268, 2099 (2001), Abstract; Full Text
MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation: A. Trockenbacher, et al.; Nat. Genet. 29, 287 (2001), Abstract; Full Text
Insulin selectively stimulates nuclear phosphoinositide-specific phospholipase C (PI-PLC) beta1 activity through a mitogen-activated protein (MAP) kinase-dependent serine phosphorylation: A.M. Martelli, et al.; FEBS Lett. 486, 230 (2000), Abstract;
The ternary complex factor Net contains two distinct elements that mediate different responses to MAP kinase signalling cascades: C. Ducret, et al.; Oncogene 19, 5063 (2000), Abstract;

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Phosphoserine detection set 

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