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Phosphoserine monoclonal antibody (4H4)

 
ALX-804-165-C100 100 µg 356.00 USD
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Replaces Prod. #: BML-SA335

Product Specification

Clone:4H4
 
Host:Mouse
 
Isotype:IgM
 
Immunogen:Phosphoserine containing peptides conjugated to KLH.
 
Species reactivity:Species independent
 
Specificity:Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with phosphoserine surrounded by positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.). Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
 
Applications:ELISA, IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Included. (Prod. No. 840-012-1)
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 1 week.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils: S.J. Gardai, et al.; J. Biol. Chem. 279, 21085 (2004), Abstract; Full Text
Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt, et al.; Proteomics 3, 1637 (2003), Abstract;
MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation: A. Trockenbacher, et al.; Nat. Genet. 29, 287 (2001), Abstract; Full Text
Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation: S. Dimmeler, et al.; Nature 399, 601 (1999), Abstract;
Insulin signaling in the yeast Saccharomyces cerevisiae. 3. Induction of protein phosphorylation by human insulin: G. Muller, et al.; Biochemistry 37, 8705 (1998), Abstract;
Insulin-mimetic signalling of synthetic phosphoinositolglycans in isolated rat adipocytes: W. Frick, et al.; Biochem. J. 336 ( Pt 1), 163 (1998), Abstract; Full Text
Characterization and purification of human corneodesmosin, an epidermal basic glycoprotein associated with corneocyte-specific modified desmosomes: M. Simon, et al.; J. Biol. Chem. 272, 31770 (1997), Abstract; Full Text

Related Products

Phosphoserine detection set 

IP, WB | Print as PDF
 
ALX-850-023-KI01 1 Set 704.00 USD
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