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United States 

Phosphoserine monoclonal antibody (1C8)

ALX-804-162-C100 100 µg 563.00 USD
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Replaces Prod. #: BML-SA332

Product Specification

Immunogen:Phosphoserine containing peptides conjugated to KLH.
Species reactivity:Species independent
Specificity:Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with phosphoserine surrounded by positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.). Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS containing 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone (PVP)
0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Included. (Prod. No. 840-012-1)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 1 week.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C

Product Literature References

Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt, et al.; Proteomics 3, 1637 (2003), Abstract;
Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites: S. Suer, et al.; Eur. J. Biochem. 268, 2099 (2001), Abstract; Full Text
Light regulation of protein phosphorylation in Blepharisma japonicum: H. Fabzak et al.; Acta Protozool. 40, 311 (2001),
Regulation of the ligand binding activity of the human very low density lipoprotein receptor by protein kinase C-dependent phosphorylation: R. Sakthivel, et al.; J. Biol. Chem. 276, 555 (2001), Abstract; Full Text
Functional regulation of metabotropic glutamate receptor type 1c: a role for phosphorylation in the desensitization of the receptor: F. Ciruela, et al.; FEBS Lett. 462, 278 (1999), Abstract;
Insulin signaling in the yeast Saccharomyces cerevisiae. 3. Induction of protein phosphorylation by human insulin: G. Muller, et al.; Biochemistry 37, 8705 (1998), Abstract;
Insulin-mimetic signalling of synthetic phosphoinositolglycans in isolated rat adipocytes: W. Frick, et al.; Biochem. J. 336, 163 (1998), Abstract; Full Text
Characterization and purification of human corneodesmosin, an epidermal basic glycoprotein associated with corneocyte-specific modified desmosomes: M. Simon, et al.; J. Biol. Chem. 272, 31770 (1997), Abstract; Full Text

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Phosphoserine detection set 

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