BAFF (mouse), mAb (5A8)



ALX-804-158-C100	100 µg	360.00 USD

Product Specification

Alternative Name:	BlyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257

Concentration:	1mg/ml

Purity:	>95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	5A8

Isotype:	Rat IgG1

Immunogen:	Rat RBL cells expressing mouse BAFF.

Specificity:	Recognizes mouse BAFF.

Application:	ELISA (capture) Flow Cytometry (excellent) Western Blot

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	Avoid freeze/thaw cycles.

Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10⁵) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1µg/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

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Product Literature References

TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004), Abstract;