Product Details
| Alternative Name: | B cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257 |
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| Clone: | 1C9 |
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| Host: | Rat |
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| Isotype: | IgG1 |
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| Immunogen: | Rat RBL cells expressing mouse BAFF. |
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| UniProt ID: | Q9WU72 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Mouse
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| Applications: | ELISA, Flow Cytometry
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| Recommended Dilutions/Conditions: | ELISA (detection)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10
5) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1µg/ml of MAb to BAFF (mouse) (1C9). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No.
ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry."
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Product Literature References
TACI, unlike BAFF-R, is solely activated by oligomeric BAFF and APRIL to support survival of activated B cells and plasmablasts: C. Bossen, et al.; Blood
111, 1004 (2008),
Abstract;
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol.
172, 812 (2004),
Abstract;
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