Product Specification
Clone: | Kimmy-2 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant mouse Tollip (aa 1-274). |
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UniProt ID: | Q9QZ06 |
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Source: | Purified from concentrated hybridoma tissue supernatant. |
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Species reactivity: | Mouse
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Crossreactivity: | Weakly cross-reacts with human tollip. |
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Applications: | ICC, IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (1:100) Western Blot (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Figure: Immunocytochemistry showing the detection of Tollip in Tollip -/- (A) or overexpressed Tollip in -/- (B) murine embryonic fibroblasts (MEF) cells with the MAb to Tollip (Kimmy-2) (Prod. No.
ALX-804-154).
Method: Following fixation with paraformaldehyde 3.7% and permeabilization with 0.2% saponin, MEF cells were incubated with the MAb to Tollip (Kimmy-2) at a 1:100 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection.
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For Research Use Only. Not for use in diagnostic procedures.