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APRIL (human) monoclonal antibody (Aprily-8)

 
ALX-804-149-C100 100 µg 323.00 USD
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Product Specification

Alternative Name:A-Proliferation-inducing ligand, TNFSF 13, CD256
 
Clone:Aprily-8
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human APRIL (aa 93-233).
 
UniProt ID:O75888
 
Source:Purified from concentrated hybridoma tissue supernatant.
 
Species reactivity:Human
 
Specificity:Recognizes APRIL and TWE-PRIL.
 
Crossreactivity:Does not cross-react with mouse APRIL or TWE-PRIL.
 
Applications:Flow Cytometry, ICC, IHC (PS), IP, WB
 
Application Notes:Excellent for Immunocytochemistry and Western Blot.
 
Positive Control:For Positive Control (Western blot) see Prod. No. ALX-840-607.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least one year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
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Figure 1: Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10µg of protein was applied to the gel. Revealed with Aprily-8 (1µg/ml) and HRP-coupled anti-mouse secondary antibody (1:4’000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).
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Figure 2: FACS analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x105) were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10µg/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.
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Figure 3: Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4% formaldehyde 5 min. at RT. After a wash in PBS, samples were dehydrated by washes in 60%, 80%, 90%, 100% EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50°C. Slides were then successively washed 2x 10 min. in xylol, 2x 10 min. in 100% ethanol, and then treated 10 min. in 100% methanol/0.6% H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90%, 80%, 60% ethanol and PBS. After micro-wave treatment, slides were washed 3x in PBS, blocked with IgG, and incubated for 1 hour with 5µg/ml Aprily-8 or control mouse IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After PBS washes, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.
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Product Literature References

Expression of TNF-superfamily members BAFF and APRIL in breast cancer: Immunohistochemical study in 52 invasive ductal breast carcinomas: V. Pelekanou, et al.; BMC Cancer 8, 76 (2008), Abstract; Full Text

General Literature References

BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. (2002), Abstract;

Related Products

Positive control (cell lysate), for antibodies to APRIL (human) 

Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human APRIL (aa 1-250) fused at the C-terminus  to a FLAG®-tag. | Print as PDF
 
ALX-840-607-R100 100 µl 48.00 USD
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