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APRIL (human) monoclonal antibody (Aprily-1)

 
ALX-804-148-C100 100 µg 330.00 USD
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Product Specification

Alternative Name:A-Proliferation-inducing ligand, TNFSF 13, CD256
 
Clone:Aprily-1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human APRIL (aa 93-233).
 
UniProt ID:O75888
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Crossreactivity:Does not cross-react with mouse APRIL.
 
Applications:Flow Cytometry, IP, WB
 
Application Notes:Flow Cytometry: also works for intracellular FC staining.
Excellent for Western Blot.
 
Positive Control:For Positive Control (Western blot) see Prod. No. ALX-840-607.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
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Figure 1: Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-1 reacts specifically with human APRIL.Method: 10µg of protein was applied to the gel. Revealed with Aprily-1 (1µg/ml) and HRP-coupled anti-mouse secondary antibody (1:4’000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).
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Figure 2: Detection of endogenous human APRIL with MAb to APRIL (Aprily-1) after IP with recombinant human BCMA:Fc.Method: Cell supernatants were first precleared with protein-A sepharose and then subjected to immunoprecipitation with rhBCMA:Fc (Prod. No. ALX-522-026) and protein-A sepharose. Beads were washed twice with PBS. Immunoprecipitated materials were eluted with 2.5mM glycine pH 2.5 for 10 min., neutralized with Tris buffer and run on an SDS-PAGE gel and analyzed with 2µg/ml Aprily-1 by Western blot. Note: In some migrations endogenous soluble APRIL resolved as a doublet around 20kDa (dendritic cells: DC). MegaAPRIL™, Soluble (human) (rec.) (Prod. No. ALX-522-035) served as a positive control.
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Figure 3:FACS analysis of cells with MAb to hAPRIL (Aprily-1).Method: HEK 293  cells were mock transfected or transfected with an expression plasmid enabling surface expression of a non-cleavable human APRIL. Cells (5x105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 10µg/ml of Aprily-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.
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Product Literature References

BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004), Abstract;

General Literature References

BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. (2002), Abstract;

Related Products

Positive control (cell lysate), for antibodies to APRIL (human) 

Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human APRIL (aa 1-250) fused at the C-terminus  to a FLAG®-tag. | Print as PDF
 
ALX-840-607-R100 100 µl 48.00 USD
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