Product Specification
| Alternative Name: | BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257 |
| |
| Concentration: | 1mg/ml |
| |
| Purity: | 95% (SDS-PAGE) |
| |
| Formulation: | Liquid. Antibody in PBS containing 0.02% sodium azide. |
| |
| Clone: | Buffy-XI |
| |
| Isotype: | Mouse IgG1 |
| |
| Immunogen: | Extracellular domain of human BAFF (aa 137-285). |
| |
| Source/Host: | Purified from concentrated hybridoma tissue culture supernatant. |
| |
| Specificity: | Recognizes C-terminus but not the stalk of human BAFF. Does not cross-react with mouse BAFF. |
| |
| Application: | Western Blot (1µg/ml) |
| |
| Short Term Storage: | +4°C |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
Figure: Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector.
Method: 10µg of total protein lysate was applied to the gel. MAb to BAFF (Buffy-XI) was used at 1µg/ml and revealed with HRP-coupled anti-mouse secondary antibody (1:4'000).
Please mouse over
General Literature References
BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol.
21, 231 (2003),
Abstract;