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 United States

BAFF (human), mAb (Buffy-XI)

 
ALX-804-147-C100 100 µg 360.00 USD
 

Product Specification

Alternative Name:BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257
 
Concentration:1mg/ml
 
Purity:95% (SDS-PAGE)
 
Formulation:Liquid. Antibody in PBS containing 0.02% sodium azide.
 
Clone:Buffy-XI
 
Isotype:Mouse IgG1
 
Immunogen:Extracellular domain of human BAFF (aa 137-285).
 
Source/Host:Purified from concentrated hybridoma tissue culture supernatant.
 
Specificity:Recognizes C-terminus but not the stalk of human BAFF. Does not cross-react with mouse BAFF.
 
Application:Western Blot (1µg/ml)
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles.
 
804-147
Figure: Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector.

Method: 10µg of total protein lysate was applied to the gel. MAb to BAFF (Buffy-XI) was used at 1µg/ml and revealed with HRP-coupled anti-mouse secondary antibody (1:4'000).

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804-147

General Literature References

BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. 21, 231 (2003), Abstract;

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