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APRIL monoclonal antibody (Sacha-1)

 
ALX-804-141-C100 100 µg 313.00 USD
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Product Specification

Alternative Name:TNFSF 13, A-Proliferation-inducing ligand, CD256
 
Clone:Sacha-1
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant human APRIL (aa 105-250) fused to human ACRP30headless (aa 16-108) (Prod. No. ALX-522-035).
 
UniProt ID:O75888
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human, Mouse
 
Specificity:Excellent for mouse April; also stains human APRIL.
 
Applications:Flow Cytometry, ICC
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
804-141
Figure 1: MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141) detects membrane-bound human APRIL by FACS.Method: HEK 293T  cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141).  After washing in FACS buffer, PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
804-141 2
Figure 2: Immunostaining of HEK-293T cells transfected with a murine APRIL expression plasmid (left panels), or mock transfected (right panel).Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5µg/ml Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.
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