APRIL, mAb (Sacha-1)



ALX-804-141-C100	100 µg	290.00 USD
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Product Specification

Alternative Name:	TNFSF 13, A-Proliferation-inducing ligand, CD256

Concentration:	1mg/ml

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	Sacha-1

Isotype:	Rat IgG2a

Immunogen:	Recombinant human APRIL (aa 105-250) fused to human ACRP30headless (aa 16-108) (Prod. No. ALX-522-035).

Source/Host:	Purified from concentrated hybridoma tissue culture supernatant.

Specificity:	Recognizes human and mouse APRIL.

Application:	Flow Cytometry (excellent for mouse APRIL, also stains human APRIL) Immunocytochemistry

Long Term Storage:	+4°C

Use/Stability:	Stable for at least 6 months after receipt when stored at +4°C undiluted.

Handling:	Do not freeze/thaw.

Figure 1: MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141) detects membrane-bound human APRIL by FACS.Method: HEK 293T cells (5x10⁵) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141). After washing in FACS buffer, PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.

Figure 2: Immunostaining of HEK-293T cells transfected with a murine APRIL expression plasmid (left panels), or mock transfected (right panel).Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5µg/ml Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

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