A20 (human), mAb (8E8.38)



ALX-804-137-C050	50 µg	360.00 USD

A20 is a zinc finger protein that renders cells resistant to apoptosis. A20-deficient mice develop severe inflammation and are hyper-responsive to LPS. S.M. O'Reilly & P.N. Moynagh have shown that full length A20 inhibits activation of NF-kB and AP-1 by TLR4. A20 associates with A20-binding inhibitor of NF-kB (ABIN)-1 and -2, which interfere with NF-kB activation upstream of the IKK complex.

Product Specification

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	8E8.38

Isotype:	Mouse IgG1

Immunogen:	Zinc finger domain corresponding to aa 375-537 of human A20.

Specificity:	Recognizes human A20.

Application:	Western Blot (1:1'000)

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored as recommended.

Handling:	Avoid freeze/thaw cycles.

Figure: Detection of human A20 by Western blot.

Method: HT1080 cells, wild type or transfected with human A20 were activated with 0.1µg/ml of rhsTNF-a (Prod. No. ALX-522-008) for 4 hours. Cells were then harvested, lysed and the cell extracts were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the 8E8.38 monoclonal antibody (1:1000 dilution O/N). Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG1 and a chemiluminescence detection system.

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Product Literature References

A20 inhibits NF-kappa B activation downstream of multiple Map3 kinases and interacts with the I kappa B signalosome: F.S. Zetoune, et al.; Cytokine 15, 282 (2001), Abstract;

General Literature References

Regulation of Toll-like receptor 4 signalling by A20 zinc finger protein: S.M. O'Reilly and P.N. Moynagh; Biochem. Biophys. Res. Commun. 3303, 586 (2003), Abstract;

Structure-function analysis of the A20-binding inhibitor of NF-kappa B activation, ABIN-1: K. Heyninck, et al.; FEBS Lett. 536, 135 (2003), Abstract;

Identification of a novel A20-binding inhibitor of nuclear factor-kappa B activation termed ABIN-2: S. Van Huffel, et al.; J Biol Chem 276, 30216 (2001), Abstract; Full Text

The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN: K. Heyninck, et al.; J. Cell Biol. 145, 1471 (1999), Abstract; Full Text