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Tollip monoclonal antibody (Kimmy-1)

 
ALX-804-132-C100 100 µg 367.00 USD
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Product Specification

Clone:Kimmy-1
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse Tollip.
 
UniProt ID:Q9QZ06
 
Species reactivity:Human, Mouse
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Western Blot (5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C undiluted.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
804-132
Figure: Detection of endogenous tollip in murine EL-4. Optimal dilution of Kimmy-1 is between 1-5µg/ml. Extracts should be made from approximately 106 -107 cells.
Method: RIPA buffer extracts from 1.5x106 EL-4 cells were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with Kimmy-1 at a dilution of 5µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (ALX-211-052) and a chemiluminescence detection system.
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804-132

Product Literature References

Threonine 66 in the death domain of IRAK-1 is critical for interaction with signaling molecules but is not a target site for autophosphorylation: D. Neumann, et al.; J. Leukoc. Biol. 84, 807 (2008), Abstract;
Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor: K. Burns, et al.; Nat. Cell Biol. 2, 346 (2000), Abstract;

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