Product Details
| Alternative Name: | B cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257 |
| |
| Clone: | Buffy-2 |
| |
| Host: | Rat |
| |
| Isotype: | IgM |
| |
| Immunogen: | Recombinant human soluble BAFF (aa 83-285) (Prod. No. ALX-522-025). |
| |
| UniProt ID: | Q9Y275 |
| |
| Source: | Purified from ascites. |
| |
| Species reactivity: | Human
|
| |
| Specificity: | Recognizes membrane-bound and soluble BAFF. |
| |
| Crossreactivity: | Weakly cross-reacts with mouse BAFF. |
| |
| Applications: | Flow Cytometry, IHC (FS), IHC (PS), WB
|
| |
| Purity Detail: | Purified by IgM purification HP column. |
| |
| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
| |
| Use/Stability: | Stable for at least 6 months after receipt when stored as recommended. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice Not Frozen |
| |
| Long Term Storage: | +4°C |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.
Figure: Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2.
Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.
Please mouse over
Product Literature References
APRIL binding to BCMA activates a JNK2-FOXO3-GADD45 pathway and induces a G2/M cell growth arrest in liver cells: G. Notas, et al.; J. Immunol.
189, 4748 (2012),
Abstract;
Detection of the TNFSF members BAFF, APRIL, TWEAK and their receptors in normal kidney and renal cell carcinomas: V. Pelekanou, et al.; Anal. Cell. Pathol. (Amst.)
34, 49 (2011),
Abstract;
Adipocytes as immune cells: differential expression of TWEAK, BAFF, and APRIL and their receptors (Fn14, BAFF-R, TACI, and BCMA) at different stages of normal and pathological adipose tissue development: V.I. Alexaki, et al.; J. Immunol.
183, 5948 (2009),
Abstract;
Systemic activation of the immune system induces aberrant BAFF and APRIL expression in B cells in patients with systemic lupus erythematosus: V.T. Chu, et al.; Arthritis Rheum.
60, 2083 (2009),
Application(s): Flow Cytometry,
Abstract;
Expression of TNF-superfamily members BAFF and APRIL in breast cancer: Immunohistochemical study in 52 invasive ductal breast carcinomas: V. Pelekanou, et al.; BMC Cancer
8, 76 (2008),
Abstract;
Full Text
TACI, unlike BAFF-R, is solely activated by oligomeric BAFF and APRIL to support survival of activated B cells and plasmablasts: C. Bossen, et al.; Blood
111, 1004 (2008),
Application(s): Western Blot ,
Abstract;
In vitro and in vivo activation induces BAFF and APRIL expression in B cells: V.T. Chu, et al.; J. Immunol.
179, 5947 (2007),
Application(s): Flow Cytometry of B Cells,
Abstract;
Full Text
Association between circulating levels of the novel TNF family members APRIL and BAFF and lymphoid organization in primary Sjogren’s syndrome: M.V. Jonsson, et al.; J. Clin. Immunol.
25, 189 (2005),
Abstract;
Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt’s lymphoma: C.A. Ogden, et al.; J. Immunol.
174, 3015 (2005),
Abstract;
Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol.
16, 467 (2004),
Abstract;
Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood
103, 689 (2004),
Abstract;
Expression of BLyS and its Receptors in B-Cell Non-Hodgkin Lymphoma: Correlation with Disease Activity and Patient Outcome: A.J. Novak, et al.; Blood
104, 2247 (2004),
Abstract;
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol.
172, 3268 (2004),
Abstract;
Full Text
Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest.
109, 59 (2002),
Abstract;
Full Text
General Literature References
Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice: S.D. Khare, et al.; PNAS
97, 3370 (2000),
Abstract;
Full Text
BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med.
189, 1747 (1999),
Abstract;
Full Text
BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator: P.A. Moore, et al.; Science
285, 260 (1999),
Abstract;
Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kB, and c-Jun NH2-terminal kinase: A. Mukhopadhyay, et al.; J. Biol. Chem.
274, 15978 (1999),
Abstract;
Full Text
Mice transgenic for BAFF develop lymphocytic disorders along with autoimmune manifestations: F. Mackay, et al.; J. Exp. Med.
190, 1697 (1999),
Abstract;
Full Text
TALL-1 is a novel member of the TNF family that is down-regulated by mitogens: H.B. Shu, et al.; J. Leukoc. Biol.
65, 680 (1999),
Abstract;
Related Products
