BAFF, mAb (Buffy-2)



ALX-804-131-C100	100 µg	340.00 USD
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Product Specification

Alternative Name:	zTNF4, THANK, TALL-1, BLyS, TALL, TNFSF 13B/20, CD257

Concentration:	1mg/ml

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	Buffy-2

Isotype:	Rat IgM

Immunogen:	Recombinant human soluble BAFF (aa 83-285) (Prod. No. ALX-522-025).

Specificity:	Recognizes membrane-bound and soluble human and mouse (weak) BAFF.

Application:	Flow Cytometry Immunohistochemistry (frozen sections, paraffin sections) Western Blot

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored as recommended.

Handling:	Do not freeze/thaw.

Positive Control:	For Positive Control (Western blot) see Prod. No. ALX-840-605.

Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.

Figure: Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2.
Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol.

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Product Literature References

Expression of TNF-superfamily members BAFF and APRIL in breast cancer: Immunohistochemical study in 52 invasive ductal breast carcinomas: V. Pelekanou, et al.; BMC Cancer 8, 76 (2008), Abstract; Full Text

In vitro and in vivo activation induces BAFF and APRIL expression in B cells: V.T. Chu, et al.; J. Immunol. 179, 5947 (2007), Abstract;

Association between circulating levels of the novel TNF family members APRIL and BAFF and lymphoid organization in primary Sjogren’s syndrome: M.V. Jonsson, et al.; J. Clin. Immunol. 25, 189 (2005), Abstract;

Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt’s lymphoma: C.A. Ogden, et al.; J. Immunol. 174, 3015 (2005), Abstract; Full Text

BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004), Abstract;

Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood 103, 689 (2004), Abstract;

Expression of BLyS and its Receptors in B-Cell Non-Hodgkin Lymphoma: Correlation with Disease Activity and Patient Outcome: A.J. Novak, et al.; Blood 104, 2247 (2004), Abstract;

Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004), Abstract; Full Text

Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002), Abstract; Full Text

General Literature References

Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice: S.D. Khare, et al.; PNAS 97, 3370 (2000), Abstract; Full Text

BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999), Abstract; Full Text

BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator: P.A. Moore, et al.; Science 285, 260 (1999), Abstract;

Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kB, and c-Jun NH2-terminal kinase: A. Mukhopadhyay, et al.; J. Biol. Chem. 274, 15978 (1999), Abstract; Full Text

Mice transgenic for BAFF develop lymphocytic disorders along with autoimmune manifestations: F. Mackay, et al.; J. Exp. Med. 190, 1697 (1999), Abstract; Full Text

TALL-1 is a novel member of the TNF family that is down-regulated by mitogens: H.B. Shu, et al.; J. Leukoc. Biol. 65, 680 (1999), Abstract;