Product Details
| Alternative Name: | B cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257 |
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| Clone: | Buffy-1 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant human soluble BAFF (aa 83-285) (Prod. No. ALX-522-025). |
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| UniProt ID: | Q9Y275 |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human
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| Specificity: | Recognizes membrane-bound BAFF. Detects endogenous protein by FC. |
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| Applications: | Flow Cytometry, ICC, WB
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 6 months after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of BAFF (human), mAb (Buffy-1) (Prod. No. ALX-804-128): Lane 1: MW marker; Lane 2: BAFF (soluble) (human), (recombinant) (Prod. No. ALX-522-025).
Schematic structure of human soluble BAFF (rec.) (aa 83-285). Recognizes the stalk region present in the membrane-bound form of hBAFF.
Membrane-bound human BAFF detected by MAb to BAFF (human) (Buffy-1) (Prod. No.
ALX-804-128). Left panel: ICC; Right panel: phase contrast. Lower panel: 293-wt control."
Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.
Method: 293T cells (5 x 10
5) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4µg/ml of MAb to BAFF (human) (Buffy-1). After washing in FACS buffer, FITC-conjugated antibody to rat IgG(
ALX-211-051) was added and cells were analyzed by flow cytometry."
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Product Literature References
BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone: J. Moreaux, et al.; Blood
103, 3148 (2004),
Abstract;
Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival: A.J. Novak, et al.; Blood
100, 2973 (2002),
Abstract;
Full Text
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med.
192, 1677 (2000),
Abstract;
BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med.
192, 129 (2000),
Abstract;
Full Text
BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med.
189, 1747 (1999),
Abstract;
General Literature References
BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med.
192, 129 (2000),
Abstract;
BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med.
189, 1747 (1999),
Abstract;
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