BAFF (human), mAb (Buffy-1)



ALX-804-128-C100	100 µg	360.00 USD
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Product Specification

Alternative Name:	zTNF4, THANK, BLyS, TALL-1, TNFSF 13B/20, CD257

Concentration:	1mg/ml

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	Buffy-1

Isotype:	Rat IgG2a

Immunogen:	Recombinant human soluble BAFF (aa 83-285) (Prod. No. ALX-522-025).

Specificity:	Recognizes membrane-bound human BAFF. Detects endogenous protein by FC.

Application:	Flow Cytometry Immunocytochemistry Western Blot Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored as recommended.

Handling:	Avoid freeze/thaw cycles.

Positive Control:	For Positive Control (Western blot) see Prod. No. ALX-840-605.

Schematic structure of human soluble BAFF (rec.) (aa 83-285). Recognizes the stalk region present in the membrane-bound form of hBAFF.

Membrane-bound human BAFF detected by MAb to BAFF (human) (Buffy-1) (Prod. No. ALX-804-128). Left panel: ICC; Right panel: phase contrast. Lower panel: 293-wt control.

Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.Method: 293T cells (5 x 10⁵) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4µg/ml of MAb to BAFF (human) (Buffy-1). After washing in FACS buffer, FITC-conjugated antibody to rat IgG(ALX-211-051) was added and cells were analyzed by flow cytometry.

Western blot analysis of BAFF (human), mAb (Buffy-1) (Prod. No. ALX-804-128): Lane 1: MW marker; Lane 2: BAFF (soluble) (human), (recombinant) (Prod. No. ALX-522-025).

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Product Literature References

BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone: J. Moreaux, et al.; Blood 103, 3148 (2004), Abstract;

Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival: A.J. Novak, et al.; Blood 100, 2973 (2002), Abstract; Full Text

A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000), Abstract;

BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000), Abstract; Full Text

BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999), Abstract;

General Literature References

BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000), Abstract;

BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999), Abstract;