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FasL (human) monoclonal antibody (Mike-2)

 
ALX-804-125-C100 100 µg 313.00 USD
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Product Specification

Alternative Name:CD95L, APO-1L, CD178, TNFSF 6
 
Clone:Mike-2
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant human soluble FasL (CD95L; APO-1L; CD178) (aa 103-281).
 
UniProt ID:P48023
 
Species reactivity:Human
 
Specificity:Recognizes only the membrane-bound active (non-cleaved) FasL and not the inactive soluble FasL.
 
Applications:Flow Cytometry, WB
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
804-125
Schematic structure of Human FasL (recombinant, aa 103-281). The antibody recognizes an epitope within aa 103-126.
804-125-1
Figure: Detection of membrane-bound FasL in HEK 293T cells transfected with a human FasL expression plasmid (right lane) compared to untransfected cells (left lane). Arrows indicate FasL and glycosylated forms of FasL.Method: Cell extracts from FasL transfected cells (5x104) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL Mike-2 antibody at 1 µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (Prod. No. ALX-211-052) and a chemiluminescence detection system.
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Product Literature References

Constitutive Expression of FasL in Thyrocytes: T.A. Stokes, et al.; Science 279, 2015a (1998), Full Text

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