Product Specification
| Alternative Name: | Cyt c (apo) |
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| Clone: | SJL2-4(apo) |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Horse cytochrome c. |
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| UniProt ID: | P00004 |
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| Species reactivity: | Human, Mouse, Rat
Goat, Monkey, Sheep
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| Applications: | WB
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at +4°C undiluted. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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Detection of cytochrome c in human cells. Cytoplasmic fraction (1), nuclear fraction (2). Cytochrome c migrates as a ~13kDa species. Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody SJL2-4 (apo) at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
Schematic structure of Horse cytochrome c.
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Product Literature References
Differential effects of two anti-apo-cytochrome c-specific monoclonal antibodies on the function of apo-cytochrome c-specific murine T cell clones: G. Corradin, et al.; J. Immunol.
133, 2915 (1984),
Abstract;
General Literature References
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell
90, 405 (1997),
Abstract;
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell
91, 479 (1997),
Abstract;
Cytochrome c: can't live with it-can't live without it: J.C. Reed; Cell
91, 559 (1997),
Abstract;
Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked: J. Yang, et al.; Science
275, 1129 (1997),
Abstract;
